SibEnzyme restriction enzymes database

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Mal I

Enzyme name Mal I
Prototype DpnI
SKU SE-E489
Turbo version Not available
High-concentration version Not available
Recognition site
5'… G(mA)▼TC …3'
3'… CT▲(mA)G …5'
Source Marinococus albus I
Optimal buffer SE-buffer MalI
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
252550755050
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of pBR322 DNA (dam-methylated) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on pBR322 DNA (dam-methylated)
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA, 50% glycerol; Store at -20°C.
Ligation After 5-fold overdigestion with enzyme more than 20% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity The enzyme cleaves only methylated DNA.
Supplied with enzyme 10 X SE-buffer MalI
Notes

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Mal I

Enzyme name Mal I
Prototype DpnI
SKU SE-E489
Turbo version Not available
High-concentration version Not available
Recognition site
5'… G(mA)▼TC …3'
3'… CT▲(mA)G …5'
Source Marinococus albus I
Optimal buffer SE-buffer MalI
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
252550755050
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of pBR322 DNA (dam-methylated) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on pBR322 DNA (dam-methylated)
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA, 50% glycerol; Store at -20°C.
Ligation After 5-fold overdigestion with enzyme more than 20% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity The enzyme cleaves only methylated DNA.
Supplied with enzyme 10 X SE-buffer MalI
Notes