SibEnzyme restriction enzymes database

Список ферментов

Bst2B I

Enzyme name Bst2B I
Prototype BsiI
SKU SE-E043
Turbo version Not available
High-concentration version Not available
Recognition site
5'… C▼TCGTG …3'
3'… GAGCA▲C …5'
Source Bacillus stearothermophilus 2B
Optimal buffer SE-buffer Y + BSA
Optimal temperature 60 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
75251025100100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 60°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.
Ligation After 10-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 60°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer Y, BSA
Notes To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Abdurashitov, M.A., Belichenko, O.A., Degtyarev, S.K. Unpublished observations (1995).

Поиск по названию

Bst2B I

Enzyme name Bst2B I
Prototype BsiI
SKU SE-E043
Turbo version Not available
High-concentration version Not available
Recognition site
5'… C▼TCGTG …3'
3'… GAGCA▲C …5'
Source Bacillus stearothermophilus 2B
Optimal buffer SE-buffer Y + BSA
Optimal temperature 60 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
75251025100100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 60°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.
Ligation After 10-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 60°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer Y, BSA
Notes To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Abdurashitov, M.A., Belichenko, O.A., Degtyarev, S.K. Unpublished observations (1995).