SibEnzyme restriction enzymes database

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Fbl I

Enzyme name Fbl I
Prototype AccI
SKU SE-E271
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GT▼mKAC …3'
3'… CAKm▲TG …5'
Source An E.coli strain that carries the cloned Fbl I gene from Flavobacterium balustinum
Optimal buffer SE-buffer Y
Optimal temperature 55 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
5075050100100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA, and 50% glycerol; Store at -20°C.
Ligation After 2-fold overdigestion with enzyme approximately 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 2 u.a. of enzyme for 16 hours at 55°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer Y
Notes
References
  1. Degtyarev, S.K. Unpublished observations.

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Fbl I

Enzyme name Fbl I
Prototype AccI
SKU SE-E271
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GT▼mKAC …3'
3'… CAKm▲TG …5'
Source An E.coli strain that carries the cloned Fbl I gene from Flavobacterium balustinum
Optimal buffer SE-buffer Y
Optimal temperature 55 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
5075050100100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA, and 50% glycerol; Store at -20°C.
Ligation After 2-fold overdigestion with enzyme approximately 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 2 u.a. of enzyme for 16 hours at 55°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer Y
Notes
References
  1. Degtyarev, S.K. Unpublished observations.