SibEnzyme restriction enzymes database

Список ферментов

Sma I

Enzyme name Sma I
Prototype SmaI
SKU SE-E177
Turbo version Available
High-concentration version Not available
Recognition site
5'… CCC▼GGG …3'
3'… GGG▲CCC …5'
Source An E.coli strain that carries the cloned Sma I gene from Serratia marcescens
Optimal buffer SE-buffer Y
Optimal temperature 25 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
000010050
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 25°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA (HindIII-digest)
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated (by using of high concentration T4 DNA Ligase and 10% PEG) and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 25°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer Y.
Notes
References
  1. Endow, S.A., Roberts, R.I., J. Mol. Biol. 112: 521-529 (1977).

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Sma I

Enzyme name Sma I
Prototype SmaI
SKU SE-E177
Turbo version Available
High-concentration version Not available
Recognition site
5'… CCC▼GGG …3'
3'… GGG▲CCC …5'
Source An E.coli strain that carries the cloned Sma I gene from Serratia marcescens
Optimal buffer SE-buffer Y
Optimal temperature 25 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
000010050
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 25°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA (HindIII-digest)
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated (by using of high concentration T4 DNA Ligase and 10% PEG) and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 25°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer Y.
Notes
References
  1. Endow, S.A., Roberts, R.I., J. Mol. Biol. 112: 521-529 (1977).