SibEnzyme restriction enzymes database

Список ферментов

Mfe I

Enzyme name Mfe I
Prototype MfeI
SKU SE-E295
Turbo version Available
High-concentration version Not available
Recognition site
5'… C▼AATTG …3'
3'… GTTAA▲C …5'
Source An E.coli strain, that carries the cloned gene Mfe I from Mycoplasma fermentans.
Optimal buffer SE-buffer B
Optimal temperature 37 °C
Inactivation temperature не инактивируется
Buffer activity
BGOWYROSE
1007510257525
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 20-fold overdigestion with enzyme 90% of the DNA fragments can be ligated with T4 DNA Ligase and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer B, BSA
Notes To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Halden, N.F., Wolf, J.B., Cross, S.L., Leonard, W.J. Clin. Res. 36: 404a (1988).

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Mfe I

Enzyme name Mfe I
Prototype MfeI
SKU SE-E295
Turbo version Available
High-concentration version Not available
Recognition site
5'… C▼AATTG …3'
3'… GTTAA▲C …5'
Source An E.coli strain, that carries the cloned gene Mfe I from Mycoplasma fermentans.
Optimal buffer SE-buffer B
Optimal temperature 37 °C
Inactivation temperature не инактивируется
Buffer activity
BGOWYROSE
1007510257525
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 20-fold overdigestion with enzyme 90% of the DNA fragments can be ligated with T4 DNA Ligase and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer B, BSA
Notes To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Halden, N.F., Wolf, J.B., Cross, S.L., Leonard, W.J. Clin. Res. 36: 404a (1988).