SibEnzyme restriction enzymes database

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BstV2 I

Enzyme name BstV2 I
Prototype BbvII
SKU SE-E297
Turbo version Available
High-concentration version Not available
Recognition site
5'… GAAGAC(N)2▼ …3'
3'… CTTCTG(N)6▲ …5'
Source Bacillus stearothermophilus V2
Optimal buffer SE-buffer Y
Optimal temperature 55 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
7575252510070
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 200 μg/ml BSA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.
Ligation After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 55°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer Y, BSA
Notes High enzyme concentration may result in star activity. To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Sinichkina, S.A., Dedkov, V.S., Degtyarev, S.K. Unpublished observations (2000). V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006

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BstV2 I

Enzyme name BstV2 I
Prototype BbvII
SKU SE-E297
Turbo version Available
High-concentration version Not available
Recognition site
5'… GAAGAC(N)2▼ …3'
3'… CTTCTG(N)6▲ …5'
Source Bacillus stearothermophilus V2
Optimal buffer SE-buffer Y
Optimal temperature 55 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
7575252510070
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 200 μg/ml BSA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.
Ligation After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 55°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer Y, BSA
Notes High enzyme concentration may result in star activity. To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Sinichkina, S.A., Dedkov, V.S., Degtyarev, S.K. Unpublished observations (2000). V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006