SibEnzyme restriction enzymes database

Список ферментов

BstKT I

Enzyme name BstKT I
Prototype MboI
SKU SE-E151
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GAT▼C …3'
3'… C▲TAG …5'
Source Bacillus stearothermophilus KT
Optimal buffer SE-buffer W
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
25507510050100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dam-) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA (dam-)
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Blocked by overlapping Dam methylation (G m ATC) GATC. Not blocked by CG methylation. Cut hemimethylated site:5`- GmATC-3` / 5`-GATC-3`
Supplied with enzyme 10 X SE-buffer W
Notes
References
  1. Nadeev A.N., Chernukhin V. A., Sevastyanova O.O., Tomilova J.E., Shinkarenko N.M., Evdokimov A.A., Degtyarev S. Kh. BstKTI, a New dam-Sensitive Neoschizomer of Restriction Endonuclease MboI, which is Able to Cleave Hemimethylated Substrate.// Biotekhnologia (Moscow), No.2, 5-10 (2006). (In Russian)

Поиск по названию

BstKT I

Enzyme name BstKT I
Prototype MboI
SKU SE-E151
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GAT▼C …3'
3'… C▲TAG …5'
Source Bacillus stearothermophilus KT
Optimal buffer SE-buffer W
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
25507510050100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dam-) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA (dam-)
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Blocked by overlapping Dam methylation (G m ATC) GATC. Not blocked by CG methylation. Cut hemimethylated site:5`- GmATC-3` / 5`-GATC-3`
Supplied with enzyme 10 X SE-buffer W
Notes
References
  1. Nadeev A.N., Chernukhin V. A., Sevastyanova O.O., Tomilova J.E., Shinkarenko N.M., Evdokimov A.A., Degtyarev S. Kh. BstKTI, a New dam-Sensitive Neoschizomer of Restriction Endonuclease MboI, which is Able to Cleave Hemimethylated Substrate.// Biotekhnologia (Moscow), No.2, 5-10 (2006). (In Russian)