SibEnzyme REBase

restriction enzymes database

Name search

Bst6 I

Enzyme name Bst6 I
Prototype Ksp632I
Known analogs Eam1104I, EarI, Ksp632I
SKU SE-E239
Turbo version Not available
High-concentration version Not available
Restriction type Type IISiType IIS enzymes recognize asymmetric DNA sequences and cleave outside the recognition site at a defined distance. They are widely used in Golden Gate assembly.
Recognition site
5'… CTCTTCN▼ …3'
3'… GAGAAG(N)4▲ …5'
Source Bacillus stearothermophilus 6
Optimal buffer SE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Optimal temperature 65 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
75755075100100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 65°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Storage at -20°C. Storage at -70°C is recommended for periods longer than 30 days..
Ligation After 2-fold overdigestion with enzyme 80% of DNA fragments can be ligated. Of these 80% can be recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 65°C.
Methylation sensitivity not tested
Reagents supplied with enzyme 10 X SE-buffer Y, BSA
Notes To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Abdurashitov, M.A., Dedkov, V.S., Kileva, E.V., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2000)
  2. V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006

Sequence search

Restriction enzyme search by DNA sequence
Only A, G, C, T are allowed. Maximum length is 50 nucleotides.

Enzyme list

Bst6 I

Enzyme name Bst6 I
Prototype Ksp632I
Known analogs Eam1104I, EarI, Ksp632I
SKU SE-E239
Turbo version Not available
High-concentration version Not available
Restriction type Type IISiType IIS enzymes recognize asymmetric DNA sequences and cleave outside the recognition site at a defined distance. They are widely used in Golden Gate assembly.
Recognition site
5'… CTCTTCN▼ …3'
3'… GAGAAG(N)4▲ …5'
Source Bacillus stearothermophilus 6
Optimal buffer SE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Optimal temperature 65 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
75755075100100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 65°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Storage at -20°C. Storage at -70°C is recommended for periods longer than 30 days..
Ligation After 2-fold overdigestion with enzyme 80% of DNA fragments can be ligated. Of these 80% can be recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 65°C.
Methylation sensitivity not tested
Reagents supplied with enzyme 10 X SE-buffer Y, BSA
Notes To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Abdurashitov, M.A., Dedkov, V.S., Kileva, E.V., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2000)
  2. V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006

Isoshizomers

Isoschizomers and neoschizomers

SE enzymeRecognition siteKnown analogsTypeRecognition site
Aat II
5'… GACGT▼C …3'
3'… C▲TGCAG …5'
Zra I
Neoschizomer
5'… GAC▼GTC …3'
3'… CTG▲CAG …5'
Acc16 I
5'… TGC▼GCA …3'
3'… ACG▲CGT …5'
AviII
FspI
MstI
NsbI
Isoschizomer
5'… TGC▼GCA …3'
3'… ACG▲CGT …5'
Acc36 I
5'… ACCTGC(N)4▼ …3'
3'… TGGACG(N)8▲ …5'
BfuAI
BspMI
BveI
Isoschizomer
5'… ACCTGC(N)4▼ …3'
3'… TGGACG(N)8▲ …5'
Acc65 I
5'… G▼GTACC …3'
3'… CCATG▲G …5'
Asp718I
Isoschizomer
5'… G▼GTACC …3'
3'… CCATG▲G …5'
Kpn I
Neoschizomer
5'… GGTAC▼C …3'
3'… C▲CATGG …5'
AccB1 I
5'… G▼GYRCC …3'
3'… CCRYG▲G …5'
BanI
BshNI
BspT107I
HgiCI
Isoschizomer
5'… G▼GYRCC …3'
3'… CCRYG▲G …5'
AccB7 I
5'… CCANNNN▼NTGG …3'
3'… GGTN▲NNNNACC …5'
PflBI
PflMI
Van91I
Isoschizomer
5'… CCANNNN▼NTGG …3'
3'… GGTN▲NNNNACC …5'
AccBS I
5'… GAG▼CGG …3'
3'… CTC▲GCC …5'
BsrBI
MbiI
Isoschizomer
5'… GAG▼CGG …3'
3'… CTC▲GCC …5'
Acl I
5'… AA▼CGTT …3'
3'… TTGC▲AA …5'
Psp1406I
Isoschizomer
5'… AA▼CGTT …3'
3'… TTGC▲AA …5'
AclW I
5'… GGATC(N)4▼ …3'
3'… CCTAG(N)5▲ …5'
AlwI
BinI
BspPI
Isoschizomer
5'… GGATC(N)4▼ …3'
3'… CCTAG(N)5▲ …5'
Aco I
5'… Y▼GGCCR …3'
3'… RCCGG▲Y …5'
CfrI
EaeI
Isoschizomer
5'… Y▼GGCCR …3'
3'… RCCGG▲Y …5'
Acs I
5'… R▼AATTY …3'
3'… YTTAA▲R …5'
ApoI
XapI
Isoschizomer
5'… R▼AATTY …3'
3'… YTTAA▲R …5'
Acu I
5'… CTGAAG(N)16▼ …3'
3'… GACTTC(N)14▲ …5'
Eco57I
Isoschizomer
5'… CTGAAG(N)16▼ …3'
3'… GACTTC(N)14▲ …5'
Afe I
5'… AGC▼GCT …3'
3'… TCG▲CGA …5'
Aor51HI
Eco47III
FunI
Isoschizomer
5'… AGC▼GCT …3'
3'… TCG▲CGA …5'
Ahl I
5'… A▼CTAGT …3'
3'… TGATC▲A …5'
BcuI
SpeI
Isoschizomer
5'… A▼CTAGT …3'
3'… TGATC▲A …5'
Ajn I
Psp6 I
5'… ▼CCWGG …3'
3'… GGWCC▲ …5'
EcoRII
PspGI
Isoschizomer
5'… ▼CCWGG …3'
3'… GGWCC▲ …5'
BptI
BseBI
Bst2U I
BstNI
BstOI
MvaI
Neoschizomer
5'… CC▼WGG …3'
3'… GGW▲CC …5'
Ama87 I
5'… C▼YCGRG …3'
3'… GRGCY▲C …5'
AvaI
BsiHKCI
BsoBI
Eco88I
NspIII
Isoschizomer
5'… C▼YCGRG …3'
3'… GRGCY▲C …5'
Apa I
5'… GGGCC▼C …3'
3'… C▲CCGGG …5'
Bsp120I
PspOM I
Neoschizomer
5'… G▼GGCCC …3'
3'… CCCGG▲G …5'
AsiG I
5'… A▼CCGGT …3'
3'… TGGCC▲A …5'
AgeI
BshTI
CspAI
PinAI
Isoschizomer
5'… A▼CCGGT …3'
3'… TGGCC▲A …5'
AsiS I
Rga I
5'… GCGAT▼CGC …3'
3'… CGC▲TAGCG …5'
SgfI
Isoschizomer
5'… GCGAT▼CGC …3'
3'… CGC▲TAGCG …5'
AspA2 I
5'… C▼CTAGG …3'
3'… GGATC▲C …5'
AvrII
BlnI
XmaJI
Isoschizomer
5'… C▼CTAGG …3'
3'… GGATC▲C …5'
AspLE I
BstHH I
5'… GCG▼C …3'
3'… C▲GCG …5'
CfoI
HhaI
Isoschizomer
5'… GCG▼C …3'
3'… C▲GCG …5'
Hin6I
HinP1I
HspA I
Neoschizomer
5'… G▼CGC …3'
3'… CGC▲G …5'
AspS9 I
5'… G▼GNCC …3'
3'… CCNG▲G …5'
AsuI
Cfr13I
PspPI
Sau96I
Isoschizomer
5'… G▼GNCC …3'
3'… CCNG▲G …5'
AsuC2 I
5'… CC▼SGG …3'
3'… GGS▲CC …5'
BcnI
CauII
NciI
Isoschizomer
5'… CC▼SGG …3'
3'… GGS▲CC …5'
AsuHP I
5'… GGTGA(N)8▼ …3'
3'… CCACT(N)7▲ …5'
HphI
Isoschizomer
5'… GGTGA(N)8▼ …3'
3'… CCACT(N)7▲ …5'
AsuNH I
5'… G▼CTAGC …3'
3'… CGATC▲G …5'
NheI
Isoschizomer
5'… G▼CTAGC …3'
3'… CGATC▲G …5'
Bmt I
Neoschizomer
5'… GCTAG▼C …3'
3'… C▲GATCG …5'
Bbv12 I
5'… GWGCW▼C …3'
3'… C▲WCGWG …5'
Alw21I
BsiHKAI
HgiAI
Isoschizomer
5'… GWGCW▼C …3'
3'… C▲WCGWG …5'
Bme18 I
5'… G▼GWCC …3'
3'… CCWG▲G …5'
AvaII
Eco47I
SinI
VpaK11BI
Isoschizomer
5'… G▼GWCC …3'
3'… CCWG▲G …5'
Bmu I
5'… ACTGGG(N)5▼ …3'
3'… TGACCC(N)4▲ …5'
BfiI
BmrI
Isoschizomer
5'… ACTGGG(N)5▼ …3'
3'… TGACCC(N)4▲ …5'
Bpm I
5'… CTGGAG(N)16▼ …3'
3'… GACCTC(N)14▲ …5'
GsuI
Isoschizomer
5'… CTGGAG(N)16▼ …3'
3'… GACCTC(N)14▲ …5'
Bpu14 I
5'… TT▼CGAA …3'
3'… AAGC▲TT …5'
AsuII
Bsp119I
BspT104I
BstBI
Csp45I
NspV
SfuI
Isoschizomer
5'… TT▼CGAA …3'
3'… AAGC▲TT …5'
Bsa29 I
5'… AT▼CGAT …3'
3'… TAGC▲TA …5'
BanIII
BseCI
BspDI
BspXI
Bsu15I
BsuTUI
ClaI
ZhoI
Isoschizomer
5'… AT▼CGAT …3'
3'… TAGC▲TA …5'
Bsc4 I
5'… CCNNNNN▼NNGG …3'
3'… GGNN▲NNNNNCC …5'
BseLI
BsiYI
BslI
Isoschizomer
5'… CCNNNNN▼NNGG …3'
3'… GGNN▲NNNNNCC …5'
Bse1 I
5'… ACTGGN▼ …3'
3'… TGAC▲CN …5'
BseNI
BsrI
BsrSI
Isoschizomer
5'… ACTGGN▼ …3'
3'… TGAC▲CN …5'
Bse3D I
5'… GCAATGNN▼ …3'
3'… CGTTAC▲NN …5'
BseMI
BsrDI
Isoschizomer
5'… GCAATGNN▼ …3'
3'… CGTTAC▲NN …5'
Bse8 I
5'… GATNN▼NNATC …3'
3'… CTANN▲NNTAG …5'
BsaBI
BseJI
MamI
Isoschizomer
5'… GATNN▼NNATC …3'
3'… CTANN▲NNTAG …5'
Bse21 I
5'… CC▼TNAGG …3'
3'… GGANT▲CC …5'
AxyI
Bsu36I
Eco81I
SauI
Isoschizomer
5'… CC▼TNAGG …3'
3'… GGANT▲CC …5'
Bse118 I
5'… R▼CCGGY …3'
3'… YGGCC▲R …5'
BsrFI
BssAI
Cfr10I
Isoschizomer
5'… R▼CCGGY …3'
3'… YGGCC▲R …5'
BseP I
5'… G▼CGCGC …3'
3'… CGCGC▲G …5'
BssHII
PauI
Isoschizomer
5'… G▼CGCGC …3'
3'… CGCGC▲G …5'
BseX3 I
5'… C▼GGCCG …3'
3'… GCCGG▲C …5'
BstZI
EagI
EclXI
Eco52I
XmaIII
Isoschizomer
5'… C▼GGCCG …3'
3'… GCCGG▲C …5'
BslF I
5'… GGGAC(N)10▼ …3'
3'… CCCTG(N)14▲ …5'
BsmFI
FaqI
Isoschizomer
5'… GGGAC(N)10▼ …3'
3'… CCCTG(N)14▲ …5'
Bso31 I
5'… GGTCTCN▼ …3'
3'… CCAGAG(N)5▲ …5'
BsaI
BspTNI
Eco31I
Isoschizomer
5'… GGTCTCN▼ …3'
3'… CCAGAG(N)5▲ …5'
Bsp13 I
5'… T▼CCGGA …3'
3'… AGGCC▲T …5'
AccIII
Aor13HI
BlfI
BseAI
BspEI
BspMII
Kpn2I
MroI
Isoschizomer
5'… T▼CCGGA …3'
3'… AGGCC▲T …5'
Bsp19 I
5'… C▼CATGG …3'
3'… GGTAC▲C …5'
NcoI
Isoschizomer
5'… C▼CATGG …3'
3'… GGTAC▲C …5'
Bsp1720 I
5'… GC▼TNAGC …3'
3'… CGANT▲CG …5'
BlpI
Bpu1102I
CelII
EspI
Isoschizomer
5'… GC▼TNAGC …3'
3'… CGANT▲CG …5'
BspAC I
5'… C▼CGC …3'
3'… GGC▲G …5'
AciI
SsiI
Isoschizomer
5'… C▼CGC …3'
3'… GGC▲G …5'
BssEC I
5'… C▼CNNGG …3'
3'… GGNNC▲C …5'
BsaJI
BseDI
SecI
Isoschizomer
5'… C▼CNNGG …3'
3'… GGNNC▲C …5'
BssNA I
5'… GTA▼TAC …3'
3'… CAT▲ATG …5'
Bst1107I
BstZ17I
Isoschizomer
5'… GTA▼TAC …3'
3'… CAT▲ATG …5'
BssT1 I
Erh I
5'… C▼CWWGG …3'
3'… GGWWC▲C …5'
Eco130I
EcoT14I
StyI
Isoschizomer
5'… C▼CWWGG …3'
3'… GGWWC▲C …5'
Bst2B I
5'… C▼TCGTG …3'
3'… GAGCA▲C …5'
BauI
BsiI
BssSI
Isoschizomer
5'… C▼TCGTG …3'
3'… GAGCA▲C …5'
Bst4C I
5'… ACN▼GT …3'
3'… TG▲NCA …5'
HpyCH4III
TaaI
Tsp4CI
Isoschizomer
5'… ACN▼GT …3'
3'… TG▲NCA …5'
Bst6 I
5'… CTCTTCN▼ …3'
3'… GAGAAG(N)4▲ …5'
Eam1104I
EarI
Ksp632I
Isoschizomer
5'… CTCTTCN▼ …3'
3'… GAGAAG(N)4▲ …5'
BstAC I
5'… GR▼CGYC …3'
3'… CYGC▲RG …5'
AcyI
BsaHI
Hin1I
Hsp92I
Isoschizomer
5'… GR▼CGYC …3'
3'… CYGC▲RG …5'
BstAF I
5'… C▼TTAAG …3'
3'… GAATT▲C …5'
AflII
BfrI
BspTI
Bst98I
MspCI
Isoschizomer
5'… C▼TTAAG …3'
3'… GAATT▲C …5'
BstAP I
5'… GCANNNN▼NTGC …3'
3'… CGTN▲NNNNACG …5'
ApaBI
Neoschizomer
5'… GCANNNNN▼TGC …3'
3'… CGT▲NNNNNACG …5'
BstAU I
5'… T▼GTACA …3'
3'… ACATG▲T …5'
Bsp1407I
BsrGI
SspBI
Isoschizomer
5'… T▼GTACA …3'
3'… ACATG▲T …5'
BstBA I
5'… YAC▼GTR …3'
3'… RTG▲CAY …5'
BsaAI
Isoschizomer
5'… YAC▼GTR …3'
3'… RTG▲CAY …5'
BstC8 I
5'… GCN▼NGC …3'
3'… CGN▲NCG …5'
Cac8I
Isoschizomer
5'… GCN▼NGC …3'
3'… CGN▲NCG …5'
BstDE I
5'… C▼TNAG …3'
3'… GANT▲C …5'
DdeI
HpyF3I
Isoschizomer
5'… C▼TNAG …3'
3'… GANT▲C …5'
BstDS I
5'… C▼CRYGG …3'
3'… GGYRC▲C …5'
BtgI
DsaI
Isoschizomer
5'… C▼CRYGG …3'
3'… GGYRC▲C …5'
BstEN I
5'… CCTNN▼NNNAGG …3'
3'… GGANNN▲NNTCC …5'
EcoNI
XagI
Isoschizomer
5'… CCTNN▼NNNAGG …3'
3'… GGANNN▲NNTCC …5'
BstF5 I
5'… GGATGNN▼ …3'
3'… CCTAC▲NN …5'
BseGI
Isoschizomer
5'… GGATGNN▼ …3'
3'… CCTAC▲NN …5'
Fok I
Neoschizomer
5'… GGATG(N)9▼ …3'
3'… CCTAC(N)13▲ …5'
BstFN I
5'… CG▼CG …3'
3'… GC▲GC …5'
AccII
Bsh1236I
BstUI
FnuDII
MvnI
Isoschizomer
5'… CG▼CG …3'
3'… GC▲GC …5'
BstH2 I
5'… RGCGC▼Y …3'
3'… Y▲CGCGR …5'
Bsp143II
HaeII
Isoschizomer
5'… RGCGC▼Y …3'
3'… Y▲CGCGR …5'
BstKT I
5'… GAT▼C …3'
3'… C▲TAG …5'
BfuCI
Bsp143I
BstMB I
DpnII
Kzo9 I
MboI
NdeII
Sau3AI
Neoschizomer
5'… ▼GATC …3'
3'… CTAG▲ …5'
Mal I
5'… G(mA)▼TC …3'
3'… CT▲(mA)G …5'
DpnI
Isoschizomer
5'… G(mA)▼TC …3'
3'… CT▲(mA)G …5'
BstMA I
5'… GTCTCN▼ …3'
3'… CAGAG(N)5▲ …5'
Alw26I
BsmAI
Isoschizomer
5'… GTCTCN▼ …3'
3'… CAGAG(N)5▲ …5'
BstMC I
5'… CGRY▼CG …3'
3'… GC▲YRGC …5'
Bsh1285I
BsiEI
McrI
Isoschizomer
5'… CGRY▼CG …3'
3'… GC▲YRGC …5'
BstMW I
5'… GCNNNNN▼NNGC …3'
3'… CGNN▲NNNNNCG …5'
HpyF10VI
MwoI
Isoschizomer
5'… GCNNNNN▼NNGC …3'
3'… CGNN▲NNNNNCG …5'
BstNS I
5'… RCATG▼Y …3'
3'… Y▲GTACR …5'
NspI
XceI
Isoschizomer
5'… RCATG▼Y …3'
3'… Y▲GTACR …5'
BstPA I
5'… GACNN▼NNGTC …3'
3'… CTGNN▲NNCAG …5'
BoxI
PshAI
Isoschizomer
5'… GACNN▼NNGTC …3'
3'… CTGNN▲NNCAG …5'
BstSC I
5'… ▼CCNGG …3'
3'… GGNCC▲ …5'
BssKI
StyD4I
Isoschizomer
5'… ▼CCNGG …3'
3'… GGNCC▲ …5'
Bme1390I
MspR9 I
ScrFI
Neoschizomer
5'… CC▼NGG …3'
3'… GGN▲CC …5'
BstSF I
5'… C▼TRYAG …3'
3'… GAYRT▲C …5'
BfmI
BpcI
SfcI
SfeI
Isoschizomer
5'… C▼TRYAG …3'
3'… GAYRT▲C …5'
BstSN I
5'… TAC▼GTA …3'
3'… ATG▲CAT …5'
Eco105I
SnaBI
Isoschizomer
5'… TAC▼GTA …3'
3'… ATG▲CAT …5'
BstV1 I
5'… GCAGC(N)8▼ …3'
3'… CGTCG(N)12▲ …5'
BbvI
BseXI
Isoschizomer
5'… GCAGC(N)8▼ …3'
3'… CGTCG(N)12▲ …5'
BstV2 I
5'… GAAGAC(N)2▼ …3'
3'… CTTCTG(N)6▲ …5'
BbsI
BbvII
BpiI
BpuAI
Isoschizomer
5'… GAAGAC(N)2▼ …3'
3'… CTTCTG(N)6▲ …5'
BstX2 I
5'… R▼GATCY …3'
3'… YCTAG▲R …5'
BstYI
MflI
PsuI
XhoII
Isoschizomer
5'… R▼GATCY …3'
3'… YCTAG▲R …5'
Bsu I
5'… GTATCC(N)6▼ …3'
3'… CATAGG(N)5▲ …5'
BciVI
Isoschizomer
5'… GTATCC(N)6▼ …3'
3'… CATAGG(N)5▲ …5'
BsuR I
Hae III
5'… GG▼CC …3'
3'… CC▲GG …5'
BshFI
BspANI
PhoI
Isoschizomer
5'… GG▼CC …3'
3'… CC▲GG …5'
Btr I
5'… CAC▼GTC …3'
3'… GTG▲CAG …5'
BmgBI
Isoschizomer
5'… CAC▼GTC …3'
3'… GTG▲CAG …5'
CciN I
5'… GC▼GGCCGC …3'
3'… CGCCGG▲CG …5'
NotI
Isoschizomer
5'… GC▼GGCCGC …3'
3'… CGCCGG▲CG …5'
Dra I
5'… TTT▼AAA …3'
3'… AAA▲TTT …5'
AhaIII
Isoschizomer
5'… TTT▼AAA …3'
3'… AAA▲TTT …5'
Dra III
5'… CACNNN▼GTG …3'
3'… GTG▲NNNCAC …5'
AdeI
Isoschizomer
5'… CACNNN▼GTG …3'
3'… GTG▲NNNCAC …5'
Dri I
5'… GACNNN▼NNGTC …3'
3'… CTGNN▲NNNCAG …5'
AhdI
AspEI
Eam1105I
EclHKI
Isoschizomer
5'… GACNNN▼NNGTC …3'
3'… CTGNN▲NNNCAG …5'
DseD I
5'… GACNNNN▼NNGTC …3'
3'… CTGNN▲NNNNCAG …5'
AasI
DrdI
Isoschizomer
5'… GACNNNN▼NNGTC …3'
3'… CTGNN▲NNNNCAG …5'
EcoICR I
5'… GAG▼CTC …3'
3'… CTC▲GAG …5'
Ecl136II
Isoschizomer
5'… GAG▼CTC …3'
3'… CTC▲GAG …5'
Psp124B I
SacI
SstI
Neoschizomer
5'… GAGCT▼C …3'
3'… C▲TCGAG …5'
EcoR I
5'… G▼AATTC …3'
3'… CTTAA▲G …5'
FunII
Isoschizomer
5'… G▼AATTC …3'
3'… CTTAA▲G …5'
EcoR V
5'… GAT▼ATC …3'
3'… CTA▲TAG …5'
Eco32I
Isoschizomer
5'… GAT▼ATC …3'
3'… CTA▲TAG …5'
Ege I
5'… GGC▼GCC …3'
3'… CCG▲CGG …5'
EheI
SfoI
Isoschizomer
5'… GGC▼GCC …3'
3'… CCG▲CGG …5'
BbeI
Neoschizomer
5'… GGCGC▼C …3'
3'… C▲CGCGG …5'
KasI
Neoschizomer
5'… G▼GCGCC …3'
3'… CCGCG▲G …5'
Mly113 I
NarI
Neoschizomer
5'… GG▼CGCC …3'
3'… CCGC▲GG …5'
Fae I
5'… CATG▼ …3'
3'… ▲GTAC …5'
Hin1II
Hsp92II
NlaIII
Isoschizomer
5'… CATG▼ …3'
3'… ▲GTAC …5'
CviAII
Neoschizomer
5'… C▼ATG …3'
3'… GTA▲C …5'
Fat I
Neoschizomer
5'… ▼CATG …3'
3'… GTAC▲ …5'
Fau I
5'… CCCGC(N)4▼ …3'
3'… GGGCG(N)6▲ …5'
SmuI
Isoschizomer
5'… CCCGC(N)4▼ …3'
3'… GGGCG(N)6▲ …5'
FauND I
5'… CA▼TATG …3'
3'… GTAT▲AC …5'
NdeI
Isoschizomer
5'… CA▼TATG …3'
3'… GTAT▲AC …5'
Fbl I
5'… GT▼mKAC …3'
3'… CAKm▲TG …5'
AccI
XmiI
Isoschizomer
5'… GT▼mKAC …3'
3'… CAKm▲TG …5'
BstF5 I
5'… GGATGNN▼ …3'
3'… CCTAC▲NN …5'
Fok I
Neoschizomer
5'… GGATG(N)9▼ …3'
3'… CCTAC(N)13▲ …5'
BseGI
Neoschizomer
5'… GGATGNN▼ …3'
3'… CCTAC▲NN …5'
FriO I
5'… GRGCY▼C …3'
3'… C▲YCGRG …5'
BanII
Eco24I
EcoT38I
HgiJII
Isoschizomer
5'… GRGCY▼C …3'
3'… C▲YCGRG …5'
Fsp4H I
5'… GC▼NGC …3'
3'… CGN▲CG …5'
Fnu4HI
ItaI
SatI
Isoschizomer
5'… GC▼NGC …3'
3'… CGN▲CG …5'
Hga I
5'… GACGC(N)5▼ …3'
3'… CTGCG(N)10▲ …5'
CseI
Isoschizomer
5'… GACGC(N)5▼ …3'
3'… CTGCG(N)10▲ …5'
Hind II
5'… GTY▼RAC …3'
3'… CAR▲YTG …5'
HincII
Isoschizomer
5'… GTY▼RAC …3'
3'… CAR▲YTG …5'
Hpa I
5'… GTT▼AAC …3'
3'… CAA▲TTG …5'
KspAI
Isoschizomer
5'… GTT▼AAC …3'
3'… CAA▲TTG …5'
Hpa II
Msp I
5'… C▼CGG …3'
3'… GGC▲C …5'
BsiSI
HapII
Isoschizomer
5'… C▼CGG …3'
3'… GGC▲C …5'
HpySE526 I
5'… A▼CGT …3'
3'… TGC▲A …5'
MaeII
Isoschizomer
5'… A▼CGT …3'
3'… TGC▲A …5'
Ksp22 I
5'… T▼GATCA …3'
3'… ACTAG▲T …5'
BclI
FbaI
Isoschizomer
5'… T▼GATCA …3'
3'… ACTAG▲T …5'
Mab I
5'… A▼CCWGGT …3'
3'… TGGWCC▲A …5'
SexAI
Isoschizomer
5'… A▼CCWGGT …3'
3'… TGGWCC▲A …5'
Mfe I
5'… C▼AATTG …3'
3'… GTTAA▲C …5'
MunI
Isoschizomer
5'… C▼AATTG …3'
3'… GTTAA▲C …5'
Mhl I
5'… GDGCH▼C …3'
3'… C▲HCGDG …5'
Bsp1286I
SduI
Isoschizomer
5'… GDGCH▼C …3'
3'… C▲HCGDG …5'
Mox20 I
5'… TGG▼CCA …3'
3'… ACC▲GGT …5'
BalI
MlsI
MluNI
MscI
Msp20I
Isoschizomer
5'… TGG▼CCA …3'
3'… ACC▲GGT …5'
MroN I
5'… G▼CCGGC …3'
3'… CGGCC▲G …5'
NgoMIV
Isoschizomer
5'… G▼CCGGC …3'
3'… CGGCC▲G …5'
NaeI
Neoschizomer
5'… GCC▼GGC …3'
3'… CGG▲CCG …5'
PdiI
Neoschizomer
5'… GCC▼GGC …3'
3'… CGG▲CCG …5'
MroX I
5'… GAANN▼NNTTC …3'
3'… CTTNN▲NNAAG …5'
Asp700I
PdmI
XmnI
Isoschizomer
5'… GAANN▼NNTTC …3'
3'… CTTNN▲NNAAG …5'
MspA1 I
5'… CmG▼CKG …3'
3'… GKC▲GmC …5'
NspBII
Isoschizomer
5'… CmG▼CKG …3'
3'… GKC▲GmC …5'
Nru I
5'… TCG▼CGA …3'
3'… AGC▲GCT …5'
Bsp68I
Isoschizomer
5'… TCG▼CGA …3'
3'… AGC▲GCT …5'
PalA I
5'… GG▼CGCGCC …3'
3'… CCGCGC▲GG …5'
AscI
AsoI
SgsI
Isoschizomer
5'… GG▼CGCGCC …3'
3'… CCGCGC▲GG …5'
Pce I
5'… AGG▼CCT …3'
3'… TCC▲GGA …5'
AatI
Eco147I
SseBI
StuI
Isoschizomer
5'… AGG▼CCT …3'
3'… TCC▲GGA …5'
Pci I
5'… A▼CATGT …3'
3'… TGTAC▲A …5'
BspLU11I
PscI
Isoschizomer
5'… A▼CATGT …3'
3'… TGTAC▲A …5'
PciS I
5'… GCTCTTCN▼ …3'
3'… CGAGAAG(N)4▲ …5'
LguI
SapI
Isoschizomer
5'… GCTCTTCN▼ …3'
3'… CGAGAAG(N)4▲ …5'
Pct I
5'… GAATGCN▼ …3'
3'… CTTAC▲GN …5'
BsaMI
BsmI
Mva1269I
Isoschizomer
5'… GAATGCN▼ …3'
3'… CTTAC▲GN …5'
Ple19 I
5'… CGAT▼CG …3'
3'… GC▲TAGC …5'
MvrI
PvuI
Isoschizomer
5'… CGAT▼CG …3'
3'… GC▲TAGC …5'
Pps I
5'… GAGTC(N)4▼ …3'
3'… CTCAG(N)5▲ …5'
PleI
Isoschizomer
5'… GAGTC(N)4▼ …3'
3'… CTCAG(N)5▲ …5'
MlyI
Neoschizomer
5'… GAGTC(N)5▼ …3'
3'… CTCAG(N)5▲ …5'
SchI
Neoschizomer
5'… GAGTC(N)5▼ …3'
3'… CTCAG(N)5▲ …5'
Pse31 I
5'… GGTCTC(N)1▼ …3'
3'… CCAGAG(N)5▲ …5'
Eco31I
Isoschizomer
5'… GGTCTC(N)1▼ …3'
3'… CCAGAG(N)5▲ …5'
PspC I
5'… CAC▼GTG …3'
3'… GTG▲CAC …5'
AcvI
BbrPI
Eco72I
PmaCI
PmlI
Isoschizomer
5'… CAC▼GTG …3'
3'… GTG▲CAC …5'
PspE I
5'… G▼GTNACC …3'
3'… CCANTG▲G …5'
BstEII
BstPI
Eco91I
EcoO65I
Isoschizomer
5'… G▼GTNACC …3'
3'… CCANTG▲G …5'
PspL I
5'… C▼GTACG …3'
3'… GCATG▲C …5'
BsiWI
Pfl23II
SplI
SunI
Isoschizomer
5'… C▼GTACG …3'
3'… GCATG▲C …5'
PspN4 I
5'… GGN▼NCC …3'
3'… CCN▲NGG …5'
BspLI
NlaIV
Isoschizomer
5'… GGN▼NCC …3'
3'… CCN▲NGG …5'
PspPP I
5'… RG▼GWCCY …3'
3'… YCCWG▲GR …5'
PpuMI
PpuXI
Psp5II
Isoschizomer
5'… RG▼GWCCY …3'
3'… YCCWG▲GR …5'
Pst I
5'… CTGCA▼G …3'
3'… G▲ACGTC …5'
BspMAI
Isoschizomer
5'… CTGCA▼G …3'
3'… G▲ACGTC …5'
PstN I
5'… CAGNNN▼CTG …3'
3'… GTC▲NNNGAC …5'
AlwNI
Isoschizomer
5'… CAGNNN▼CTG …3'
3'… GTC▲NNNGAC …5'
Rig I
5'… GGCCGG▼CC …3'
3'… CC▲GGCCGG …5'
FseI
Isoschizomer
5'… GGCCGG▼CC …3'
3'… CC▲GGCCGG …5'
Rsa I
5'… GT▼AC …3'
3'… CA▲TG …5'
AfaI
Isoschizomer
5'… GT▼AC …3'
3'… CA▲TG …5'
Csp6I
Neoschizomer
5'… G▼TAC …3'
3'… CAT▲G …5'
Rsr2 I
5'… CG▼GWCCG …3'
3'… GCCWG▲GC …5'
CpoI
CspI
RsrII
Isoschizomer
5'… CG▼GWCCG …3'
3'… GCCWG▲GC …5'
Sbf I
5'… CCTGCA▼GG …3'
3'… GG▲ACGTCC …5'
SdaI
Sse8387I
Isoschizomer
5'… CCTGCA▼GG …3'
3'… GG▲ACGTCC …5'
SfaN I
5'… GCATC(N)5▼ …3'
3'… CGTAG(N)9▲ …5'
LweI
Isoschizomer
5'… GCATC(N)5▼ …3'
3'… CGTAG(N)9▲ …5'
Sfr274 I
5'… C▼TCGAG …3'
3'… GAGCT▲C …5'
BssHI
PaeR7I
SlaI
StrI
TliI
XhoI
Isoschizomer
5'… C▼TCGAG …3'
3'… GAGCT▲C …5'
Sfr303 I
5'… CCGC▼GG …3'
3'… GG▲CGCC …5'
Cfr42I
KspI
SacII
SgrBI
SstII
Isoschizomer
5'… CCGC▼GG …3'
3'… GG▲CGCC …5'
Sma I
5'… CCC▼GGG …3'
3'… GGG▲CCC …5'
Cfr9I
XmaCI
Xma I
Neoschizomer
5'… C▼CCGGG …3'
3'… GGGCC▲C …5'
Smi I
5'… ATTT▼AAAT …3'
3'… TAAA▲TTTA …5'
SwaI
Isoschizomer
5'… ATTT▼AAAT …3'
3'… TAAA▲TTTA …5'
SmiM I
5'… CAYNN▼NNRTG …3'
3'… GTRNN▲NNYAC …5'
MslI
Isoschizomer
5'… CAYNN▼NNRTG …3'
3'… GTRNN▲NNYAC …5'
Sph I
5'… GCATG▼C …3'
3'… C▲GTACG …5'
BbuI
PaeI
SpaHI
Isoschizomer
5'… GCATG▼C …3'
3'… C▲GTACG …5'
Sse9 I
5'… ▼AATT …3'
3'… TTAA▲ …5'
TasI
Tsp509I
TspEI
Isoschizomer
5'… ▼AATT …3'
3'… TTAA▲ …5'
SspM I
5'… C▼TAG …3'
3'… GAT▲C …5'
MaeI
Isoschizomer
5'… C▼TAG …3'
3'… GAT▲C …5'
Tru9 I
5'… T▼TAA …3'
3'… AAT▲T …5'
MseI
Tru1I
Isoschizomer
5'… T▼TAA …3'
3'… AAT▲T …5'
TseF I
5'… ▼GTSAC …3'
3'… CASTG▲ …5'
Tsp45I
Isoschizomer
5'… ▼GTSAC …3'
3'… CASTG▲ …5'
Tth111 I
5'… GACN▼NNGTC …3'
3'… CTGNN▲NCAG …5'
AspI
PflFI
PsyI
TelI
Isoschizomer
5'… GACN▼NNGTC …3'
3'… CTGNN▲NCAG …5'
Vne I
5'… G▼TGCAC …3'
3'… CACGT▲G …5'
Alw44I
ApaLI
Isoschizomer
5'… G▼TGCAC …3'
3'… CACGT▲G …5'
Vsp I
5'… AT▼TAAT …3'
3'… TAAT▲TA …5'
AseI
PshBI
Isoschizomer
5'… AT▼TAAT …3'
3'… TAAT▲TA …5'
Zrm I
5'… AGT▼ACT …3'
3'… TCA▲TGA …5'
AssI
ScaI
Isoschizomer
5'… AGT▼ACT …3'
3'… TCA▲TGA …5'
Zsp2 I
5'… ATGCA▼T …3'
3'… T▲ACGTA …5'
EcoT22I
Mph1103I
NsiI
Isoschizomer
5'… ATGCA▼T …3'
3'… T▲ACGTA …5'
BfrBI
Neoschizomer
5'… ATGCA▼T …3'
3'… T▲ACGTA …5'
// Код для некролога