SibEnzyme restriction enzymes database

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Bse1 I

Enzyme name Bse1 I
Prototype BsrI
SKU SE-E035
Turbo version Not available
High-concentration version Not available
Recognition site
5'… ACTGGN▼ …3'
3'… TGAC▲CN …5'
Source Bacillus stearothermophilus 1
Optimal buffer SE-buffer Y
Optimal temperature 65 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
75752510100100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 65°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 20-fold overdigestion with enzyme >95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 65°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer Y
Notes
References
  1. Repin, V.E., Degtyarev, S.Kh. Unpublished observations.

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Bse1 I

Enzyme name Bse1 I
Prototype BsrI
SKU SE-E035
Turbo version Not available
High-concentration version Not available
Recognition site
5'… ACTGGN▼ …3'
3'… TGAC▲CN …5'
Source Bacillus stearothermophilus 1
Optimal buffer SE-buffer Y
Optimal temperature 65 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
75752510100100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 65°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 20-fold overdigestion with enzyme >95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 65°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer Y
Notes
References
  1. Repin, V.E., Degtyarev, S.Kh. Unpublished observations.