SibEnzyme restriction enzymes database

Список ферментов

Pst I

Enzyme name Pst I
Prototype PstI
SKU SE-E109
Turbo version Available
High-concentration version Available
Recognition site
5'… CTGCA▼G …3'
3'… G▲ACGTC …5'
Source An E.coli strain that carries the cloned Pst I gene from Providencia stuartii
Optimal buffer SE-buffer O
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
1025100252550
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 20-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer O, BSA (except E109T and E110T).
Notes High enzyme concentration may result in star activity. To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Smith, D.I., Blattner, F.R., Davies, J. Nucleic Acids Res. 3: 343-353 (1976).

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Pst I

Enzyme name Pst I
Prototype PstI
SKU SE-E109
Turbo version Available
High-concentration version Available
Recognition site
5'… CTGCA▼G …3'
3'… G▲ACGTC …5'
Source An E.coli strain that carries the cloned Pst I gene from Providencia stuartii
Optimal buffer SE-buffer O
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
1025100252550
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 20-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer O, BSA (except E109T and E110T).
Notes High enzyme concentration may result in star activity. To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Smith, D.I., Blattner, F.R., Davies, J. Nucleic Acids Res. 3: 343-353 (1976).