SibEnzyme restriction enzymes database

Список ферментов

Dra I

Enzyme name Dra I
Prototype AhaIII
SKU SE-E055
Turbo version Available
High-concentration version Not available
Recognition site
5'… TTT▼AAA …3'
3'… AAA▲TTT …5'
Source Deinococcus radiophilus
Optimal buffer SE-buffer G
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
75100257575100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA, 50% glycerol; Store at -20°C.
Ligation After 20-fold overdigestion with enzyme about 70% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer G, BSA
Notes To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Purvis, I.J., Moseley, B.E.B., Nucleic Acids Res. 11:5467-5474 (1983)

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Dra I

Enzyme name Dra I
Prototype AhaIII
SKU SE-E055
Turbo version Available
High-concentration version Not available
Recognition site
5'… TTT▼AAA …3'
3'… AAA▲TTT …5'
Source Deinococcus radiophilus
Optimal buffer SE-buffer G
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
75100257575100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA, 50% glycerol; Store at -20°C.
Ligation After 20-fold overdigestion with enzyme about 70% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer G, BSA
Notes To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Purvis, I.J., Moseley, B.E.B., Nucleic Acids Res. 11:5467-5474 (1983)