SibEnzyme restriction enzymes database

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Ksp22 I

Enzyme name Ksp22 I
Prototype BclI
SKU SE-E081
Turbo version Not available
High-concentration version Not available
Recognition site
5'… T▼GATCA …3'
3'… ACTAG▲T …5'
Source Kurthia species 22
Optimal buffer SE-buffer 2K
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
5075505025100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA, 50% glycerol; Store at -20°C.
Ligation After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Blocked by Dam methylation (G m ATC) T GATC A
Supplied with enzyme 10 X SE-buffer 2K, BSA
Notes To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Dedkov, V,S., Degtyarev, S.Kh., Unpublished observations. V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006

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Ksp22 I

Enzyme name Ksp22 I
Prototype BclI
SKU SE-E081
Turbo version Not available
High-concentration version Not available
Recognition site
5'… T▼GATCA …3'
3'… ACTAG▲T …5'
Source Kurthia species 22
Optimal buffer SE-buffer 2K
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
5075505025100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA, 50% glycerol; Store at -20°C.
Ligation After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Blocked by Dam methylation (G m ATC) T GATC A
Supplied with enzyme 10 X SE-buffer 2K, BSA
Notes To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Dedkov, V,S., Degtyarev, S.Kh., Unpublished observations. V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006