SibEnzyme restriction enzymes database

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AsuHP I

Enzyme name AsuHP I
Prototype HphI
SKU SE-E231
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GGTGA(N)8▼ …3'
3'… CCACT(N)7▲ …5'
Source An E.coli strain that carries the cloned AsuHPI gene from Actinobacillus suis HP
Optimal buffer SE-buffer O
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
10501007525100
Unit definition One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl.
Assayed on Lambda DNA (dam-)
Storage conditions 10 mM Tris-HCl (pH 7.5), 250 mM NaCl, 0.1 mM EDTA, 7 mM 2-mercaptoethanol, 200 µg/ml BSA, 50% glycerol; Store at -20°С.
Ligation After 5-fold overdigestion with AsuHP I, ~30% of the DNA fragments can be ligated with T4 DNA Ligase and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Blocked by overlapping dam-methylation (GmATC) GGT GA TC
Supplied with enzyme 10 x SE-Buffer O
Notes enzyme may cleave at N9/N8 depending on the sequence between the ecognition and cleave sites.
References
  1. Dedkov, V.S., Degtyarev, S.Kh. Biol. Chem. 379: 573-574 (1998) Murat A Abdurashitov, Victor N Tomilov, Valery A Chernukhin, S. Kh. Degtyarev A physical map of human Alu repeats cleavage by restriction endonucleases // BMC Genomics 2008, 9:305 Abdurashitov M.A., Tomilov V.N., Chernukhin V.A., Gonchar D. A., Degtyarev S. Kh. Comparative analysis of human chromosomal DNA digestion with restriction endonucleases in vitro and in silico. // "Medical genetics" V.6, No 8, pp 29-36, 2007

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AsuHP I

Enzyme name AsuHP I
Prototype HphI
SKU SE-E231
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GGTGA(N)8▼ …3'
3'… CCACT(N)7▲ …5'
Source An E.coli strain that carries the cloned AsuHPI gene from Actinobacillus suis HP
Optimal buffer SE-buffer O
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
10501007525100
Unit definition One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl.
Assayed on Lambda DNA (dam-)
Storage conditions 10 mM Tris-HCl (pH 7.5), 250 mM NaCl, 0.1 mM EDTA, 7 mM 2-mercaptoethanol, 200 µg/ml BSA, 50% glycerol; Store at -20°С.
Ligation After 5-fold overdigestion with AsuHP I, ~30% of the DNA fragments can be ligated with T4 DNA Ligase and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Blocked by overlapping dam-methylation (GmATC) GGT GA TC
Supplied with enzyme 10 x SE-Buffer O
Notes enzyme may cleave at N9/N8 depending on the sequence between the ecognition and cleave sites.
References
  1. Dedkov, V.S., Degtyarev, S.Kh. Biol. Chem. 379: 573-574 (1998) Murat A Abdurashitov, Victor N Tomilov, Valery A Chernukhin, S. Kh. Degtyarev A physical map of human Alu repeats cleavage by restriction endonucleases // BMC Genomics 2008, 9:305 Abdurashitov M.A., Tomilov V.N., Chernukhin V.A., Gonchar D. A., Degtyarev S. Kh. Comparative analysis of human chromosomal DNA digestion with restriction endonucleases in vitro and in silico. // "Medical genetics" V.6, No 8, pp 29-36, 2007