SibEnzyme restriction enzymes database

Список ферментов

Dra III

Enzyme name Dra III
Prototype DraIII
SKU SE-E309
Turbo version Available
High-concentration version Not available
Recognition site
5'… CACNNN▼GTG …3'
3'… GTG▲NNNCAC …5'
Source An E.coli strain, that carries the cloned gene Dra III from Deinococcus radiophilus
Optimal buffer SE-buffer 2K
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
2550757550100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 300 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 10-fold overdigestion with enzyme 70% of the DNA fragments can be ligated and recut. In the presence of 10%PEG
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of DNA with 5 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer 2K, BSA
Notes High enzyme concentration may result in star activity. To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. de Wit, C.M., Dekker, B.M.M., Neele, A.C., de Waard, A., FEBS Lett. 180: 219-223 (1985)Grosskopf, R., Wolf, W., Kessler, C., Nucleic Acid res. 13: 1517-1528 (1985)

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Dra III

Enzyme name Dra III
Prototype DraIII
SKU SE-E309
Turbo version Available
High-concentration version Not available
Recognition site
5'… CACNNN▼GTG …3'
3'… GTG▲NNNCAC …5'
Source An E.coli strain, that carries the cloned gene Dra III from Deinococcus radiophilus
Optimal buffer SE-buffer 2K
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
2550757550100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 300 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 10-fold overdigestion with enzyme 70% of the DNA fragments can be ligated and recut. In the presence of 10%PEG
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of DNA with 5 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer 2K, BSA
Notes High enzyme concentration may result in star activity. To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. de Wit, C.M., Dekker, B.M.M., Neele, A.C., de Waard, A., FEBS Lett. 180: 219-223 (1985)Grosskopf, R., Wolf, W., Kessler, C., Nucleic Acid res. 13: 1517-1528 (1985)