SibEnzyme restriction enzymes database

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BstMA I

Enzyme name BstMA I
Prototype BsmAI
SKU SE-E291
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GTCTCN▼ …3'
3'… CAGAG(N)5▲ …5'
Source Bacillus stearothermophilus MA
Optimal buffer SE-buffer W + BSA
Optimal temperature 55 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
25505010075100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 50-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 100 u.a. of enzyme for 16 hours at 55°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer W, BSA
Notes To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Chernukhin, V.A., Mikhnenkova, N.A., Kileva, E.V., Mezentseva, N.V., Dedkov, V.S., Degtyarev, S.K. Unpublished observations (2005) Murat A Abdurashitov, Victor N Tomilov, Valery A Chernukhin, S. Kh. Degtyarev A physical map of human Alu repeats cleavage by restriction endonucleases // BMC Genomics 2008, 9:305 Abdurashitov M.A., Tomilov V.N., Chernukhin V.A., Gonchar D. A., Degtyarev S. Kh. Comparative analysis of human chromosomal DNA digestion with restriction endonucleases in vitro and in silico. // Translated from "Medical genetics" V.6, No 8, pp 29-36, 2007

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BstMA I

Enzyme name BstMA I
Prototype BsmAI
SKU SE-E291
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GTCTCN▼ …3'
3'… CAGAG(N)5▲ …5'
Source Bacillus stearothermophilus MA
Optimal buffer SE-buffer W + BSA
Optimal temperature 55 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
25505010075100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 50-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 100 u.a. of enzyme for 16 hours at 55°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer W, BSA
Notes To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Chernukhin, V.A., Mikhnenkova, N.A., Kileva, E.V., Mezentseva, N.V., Dedkov, V.S., Degtyarev, S.K. Unpublished observations (2005) Murat A Abdurashitov, Victor N Tomilov, Valery A Chernukhin, S. Kh. Degtyarev A physical map of human Alu repeats cleavage by restriction endonucleases // BMC Genomics 2008, 9:305 Abdurashitov M.A., Tomilov V.N., Chernukhin V.A., Gonchar D. A., Degtyarev S. Kh. Comparative analysis of human chromosomal DNA digestion with restriction endonucleases in vitro and in silico. // Translated from "Medical genetics" V.6, No 8, pp 29-36, 2007