SibEnzyme restriction enzymes database

Список ферментов

BstV1 I

Enzyme name BstV1 I
Prototype BbvI
SKU SE-E303
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GCAGC(N)8▼ …3'
3'… CGTCG(N)12▲ …5'
Source Bacillus stearothermophilus V1
Optimal buffer SE-buffer G
Optimal temperature 55 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
75100757575100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of pBR322 DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed on pBR322 DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.
Ligation After 3-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of DNA with 2 u.a. of enzyme for 16 hours at 55°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer G
Notes At 37°C activity is 10% from maximum.
References
  1. Dedkov, V.S., Muradov, S.V., Shinkarenko, N.M., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2001).

Поиск по названию

BstV1 I

Enzyme name BstV1 I
Prototype BbvI
SKU SE-E303
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GCAGC(N)8▼ …3'
3'… CGTCG(N)12▲ …5'
Source Bacillus stearothermophilus V1
Optimal buffer SE-buffer G
Optimal temperature 55 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
75100757575100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of pBR322 DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed on pBR322 DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.
Ligation After 3-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of DNA with 2 u.a. of enzyme for 16 hours at 55°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer G
Notes At 37°C activity is 10% from maximum.
References
  1. Dedkov, V.S., Muradov, S.V., Shinkarenko, N.M., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2001).