SibEnzyme restriction enzymes database

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Bse21 I

Enzyme name Bse21 I
Prototype SauI
SKU SE-E037
Turbo version Not available
High-concentration version Not available
Recognition site
5'… CC▼TNAGG …3'
3'… GGANT▲CC …5'
Source Bacillus species 21
Optimal buffer SE-buffer Y
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
5050102510040
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA (HindIII-digest)
Storage conditions 10 mM KH 2 PO 4 (pH 7.4); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 2-fold overdigestion with enzyme about 50% of DNA fragments can be ligated by using of high concentration T4 DNA ligase with presence of 10% PEG. Of these more than 90% can be recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer Y
Notes
References
  1. Degtyarev, S.Kh., Rechkunova, N.I., Repin, V.E., Kolyhalov, A.A., Netesov, S.V. Izv. Sib. Otd. Akad. Nauk SSSR 1: 138-139 (1990). V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006

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Bse21 I

Enzyme name Bse21 I
Prototype SauI
SKU SE-E037
Turbo version Not available
High-concentration version Not available
Recognition site
5'… CC▼TNAGG …3'
3'… GGANT▲CC …5'
Source Bacillus species 21
Optimal buffer SE-buffer Y
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
5050102510040
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA (HindIII-digest)
Storage conditions 10 mM KH 2 PO 4 (pH 7.4); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 2-fold overdigestion with enzyme about 50% of DNA fragments can be ligated by using of high concentration T4 DNA ligase with presence of 10% PEG. Of these more than 90% can be recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer Y
Notes
References
  1. Degtyarev, S.Kh., Rechkunova, N.I., Repin, V.E., Kolyhalov, A.A., Netesov, S.V. Izv. Sib. Otd. Akad. Nauk SSSR 1: 138-139 (1990). V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006