SibEnzyme restriction enzymes database

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Vsp I

Enzyme name Vsp I
Prototype VspI
SKU SE-E139
Turbo version Available
High-concentration version Not available
Recognition site
5'… AT▼TAAT …3'
3'… TAAT▲TA …5'
Source An E.coli strain, that carries the cloned gene VspI from Vibrio species 343
Optimal buffer SE-buffer W
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
010501002550
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 10-fold overdigestion with enzyme 70% of the DNA fragments can be ligated.Of these, 90% can be recut. In the presence of 10% PEG
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C
Methylation sensitivity Blocked by ATTA m AT methylation.
Supplied with enzyme 10 X SE-buffer W
Notes
References
  1. Degtyarev, S.Kh., Repin, V.E., Rechkunova, N.I., Tchigikov, V.E., Malygin, E.G., Mikhajlov, V.V., Rasskazov, V.A. Bioorg. Khim. 13: 420-421 (1987). V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006

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Vsp I

Enzyme name Vsp I
Prototype VspI
SKU SE-E139
Turbo version Available
High-concentration version Not available
Recognition site
5'… AT▼TAAT …3'
3'… TAAT▲TA …5'
Source An E.coli strain, that carries the cloned gene VspI from Vibrio species 343
Optimal buffer SE-buffer W
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
010501002550
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 10-fold overdigestion with enzyme 70% of the DNA fragments can be ligated.Of these, 90% can be recut. In the presence of 10% PEG
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C
Methylation sensitivity Blocked by ATTA m AT methylation.
Supplied with enzyme 10 X SE-buffer W
Notes
References
  1. Degtyarev, S.Kh., Repin, V.E., Rechkunova, N.I., Tchigikov, V.E., Malygin, E.G., Mikhajlov, V.V., Rasskazov, V.A. Bioorg. Khim. 13: 420-421 (1987). V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006