SibEnzyme restriction enzymes database

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Psp124B I

Enzyme name Psp124B I
Prototype SacI
SKU SE-E107
Turbo version Available
High-concentration version Not available
Recognition site
5'… GAGCT▼C …3'
3'… C▲TCGAG …5'
Source Pseudomonas species 124B
Optimal buffer SE-buffer G
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
751001007530
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA (HindIII-digest)
Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer G.
Notes
References
  1. Degtyarev, S.Kh., Dedkov, V.S., Rechkunova, N.I., Morgan, R. Unpublished observations (1994).

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Psp124B I

Enzyme name Psp124B I
Prototype SacI
SKU SE-E107
Turbo version Available
High-concentration version Not available
Recognition site
5'… GAGCT▼C …3'
3'… C▲TCGAG …5'
Source Pseudomonas species 124B
Optimal buffer SE-buffer G
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
751001007530
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA (HindIII-digest)
Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer G.
Notes
References
  1. Degtyarev, S.Kh., Dedkov, V.S., Rechkunova, N.I., Morgan, R. Unpublished observations (1994).