SibEnzyme restriction enzymes database

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BsuR I

Enzyme name BsuR I
Prototype HaeIII
SKU SE-E053
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GG▼CC …3'
3'… CC▲GG …5'
Source Bacillus subtilis R
Optimal buffer SE-buffer G
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
75100255050100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 200 μg/ml BSA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.
Ligation After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer G
Notes
References
  1. Bron, S., Murray, K., Trautner, T.A. Mol. Gen. Genet. 143: 13-23 (1975)

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BsuR I

Enzyme name BsuR I
Prototype HaeIII
SKU SE-E053
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GG▼CC …3'
3'… CC▲GG …5'
Source Bacillus subtilis R
Optimal buffer SE-buffer G
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
75100255050100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 200 μg/ml BSA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.
Ligation After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer G
Notes
References
  1. Bron, S., Murray, K., Trautner, T.A. Mol. Gen. Genet. 143: 13-23 (1975)