SibEnzyme restriction enzymes database

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MroN I

Enzyme name MroN I
Prototype (NaeI)
SKU SE-E087
Turbo version Not available
High-concentration version Not available
Recognition site
5'… G▼CCGGC …3'
3'… CGGCC▲G …5'
Source Micrococcus roseus N0
Optimal buffer SE-buffer B
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
10050100105
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Adenovirus-2 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Adenovirus-2 DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 5-fold overdigestion with enzyme more than 90% of the Ad-2 DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Ad2 DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer B
Notes
References
  1. Dedkov, V.S., Belichenko, O.A., Prikhod'ko, E.A., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations (1994).

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MroN I

Enzyme name MroN I
Prototype (NaeI)
SKU SE-E087
Turbo version Not available
High-concentration version Not available
Recognition site
5'… G▼CCGGC …3'
3'… CGGCC▲G …5'
Source Micrococcus roseus N0
Optimal buffer SE-buffer B
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
10050100105
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Adenovirus-2 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Adenovirus-2 DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 5-fold overdigestion with enzyme more than 90% of the Ad-2 DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Ad2 DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer B
Notes
References
  1. Dedkov, V.S., Belichenko, O.A., Prikhod'ko, E.A., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations (1994).