SibEnzyme restriction enzymes database

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Dri I

Enzyme name Dri I
Prototype Eam1105I
SKU SE-E193
Turbo version Available
High-concentration version Not available
Recognition site
5'… GACNNN▼NNGTC …3'
3'… CTGNN▲NNNCAG …5'
Source Deinococcus radiodurans EA
Optimal buffer SE-buffer Y
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
7575101010040
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20°C.
Ligation After 5-fold overdigestion with enzyme about 5% of the DNA fragments can be ligated.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer Y
Notes
References
  1. Abdurashitov, M.A., Nayakshina, T.N., Dedkov, V.S., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2003)

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Dri I

Enzyme name Dri I
Prototype Eam1105I
SKU SE-E193
Turbo version Available
High-concentration version Not available
Recognition site
5'… GACNNN▼NNGTC …3'
3'… CTGNN▲NNNCAG …5'
Source Deinococcus radiodurans EA
Optimal buffer SE-buffer Y
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
7575101010040
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20°C.
Ligation After 5-fold overdigestion with enzyme about 5% of the DNA fragments can be ligated.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer Y
Notes
References
  1. Abdurashitov, M.A., Nayakshina, T.N., Dedkov, V.S., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2003)