SibEnzyme restriction enzymes database

Список ферментов

Bsp1720 I

Enzyme name Bsp1720 I
Prototype EspI
SKU SE-E185
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GC▼TNAGC …3'
3'… CGANT▲CG …5'
Source Bacillus species 1720
Optimal buffer SE-buffer G
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
5010050507575
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.
Ligation After 10-fold overdigestion with enzyme about 80% of DNA fragment can be ligated. Of these about 95% can be recut
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer G
Notes
References
  1. Degtyarev, S.Kh., Kolyhalov, A.A., Rechkunova, N.T., Tepavicharova, I.I., Mechandjiska, L.I., Builieva, E.I. Nucleic Acids Res. 19: 2504 (1991).

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Bsp1720 I

Enzyme name Bsp1720 I
Prototype EspI
SKU SE-E185
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GC▼TNAGC …3'
3'… CGANT▲CG …5'
Source Bacillus species 1720
Optimal buffer SE-buffer G
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
5010050507575
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.
Ligation After 10-fold overdigestion with enzyme about 80% of DNA fragment can be ligated. Of these about 95% can be recut
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer G
Notes
References
  1. Degtyarev, S.Kh., Kolyhalov, A.A., Rechkunova, N.T., Tepavicharova, I.I., Mechandjiska, L.I., Builieva, E.I. Nucleic Acids Res. 19: 2504 (1991).