SibEnzyme restriction enzymes database

Список ферментов

TseF I

Enzyme name TseF I
Prototype Tsp45I
SKU SE-E589
Turbo version Not available
High-concentration version Not available
Recognition site
5'… ▼GTSAC …3'
3'… CASTG▲ …5'
Source An E.coli strain, that carries the cloned gene TseFI from Thermus species F35
Optimal buffer SE-buffer B
Optimal temperature 65 °C
Inactivation temperature не инактивируется
Buffer activity
BGOWYROSE
100500255050
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 65°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 10-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 65°C.
Methylation sensitivity
Supplied with enzyme 10 X SE-buffer B
Notes

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TseF I

Enzyme name TseF I
Prototype Tsp45I
SKU SE-E589
Turbo version Not available
High-concentration version Not available
Recognition site
5'… ▼GTSAC …3'
3'… CASTG▲ …5'
Source An E.coli strain, that carries the cloned gene TseFI from Thermus species F35
Optimal buffer SE-buffer B
Optimal temperature 65 °C
Inactivation temperature не инактивируется
Buffer activity
BGOWYROSE
100500255050
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 65°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 10-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 65°C.
Methylation sensitivity
Supplied with enzyme 10 X SE-buffer B
Notes