SibEnzyme restriction enzymes database

Список ферментов

Sfr274 I

Enzyme name Sfr274 I
Prototype XhoI
SKU SE-E125
Turbo version Available
High-concentration version Not available
Recognition site
5'… C▼TCGAG …3'
3'… GAGCT▲C …5'
Source Streptomyces fradiae 274
Optimal buffer SE-buffer B
Optimal temperature 50 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
10075505075100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (Hind III-digest) in 1 hour at 50°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA (Hind III-digest)
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol.. Store at -20°C.
Ligation After 20-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 50°C.
Methylation sensitivity Blocked by CTCG m AG methylation. Not blocked by CT m CGAG methylation.
Supplied with enzyme 10 X SE-buffer B.
Notes Incubation at 37°C results in 70% activity
References
  1. Puchkova, L.I., Krivopalova, G.N., Andreeva, I.S., Selina, A.V., Serov, G.D., Rechkunova, N.I., Degtyarev, S.Kh. Izv. Sib. Otd. Akad. Nauk SSSR 1: 32-34 (1990).

Поиск по названию

Sfr274 I

Enzyme name Sfr274 I
Prototype XhoI
SKU SE-E125
Turbo version Available
High-concentration version Not available
Recognition site
5'… C▼TCGAG …3'
3'… GAGCT▲C …5'
Source Streptomyces fradiae 274
Optimal buffer SE-buffer B
Optimal temperature 50 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
10075505075100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (Hind III-digest) in 1 hour at 50°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA (Hind III-digest)
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol.. Store at -20°C.
Ligation After 20-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 50°C.
Methylation sensitivity Blocked by CTCG m AG methylation. Not blocked by CT m CGAG methylation.
Supplied with enzyme 10 X SE-buffer B.
Notes Incubation at 37°C results in 70% activity
References
  1. Puchkova, L.I., Krivopalova, G.N., Andreeva, I.S., Selina, A.V., Serov, G.D., Rechkunova, N.I., Degtyarev, S.Kh. Izv. Sib. Otd. Akad. Nauk SSSR 1: 32-34 (1990).