SibEnzyme restriction enzymes database

Список ферментов

AsuNH I

Enzyme name AsuNH I
Prototype NheI
SKU SE-E063
Turbo version Available
High-concentration version Not available
Recognition site
5'… G▼CTAGC …3'
3'… CGATC▲G …5'
Source Actinobacillus suis NH
Optimal buffer SE-buffer Y + BSA
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
75500010025
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA (HindIII-digest)
Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol. Store at -20°C
Ligation After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer Y, BSA
Notes To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Dedkov, V.S., Degtyarev, S.Kh. Biol. Chem. 379: 573-574 (1998).

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AsuNH I

Enzyme name AsuNH I
Prototype NheI
SKU SE-E063
Turbo version Available
High-concentration version Not available
Recognition site
5'… G▼CTAGC …3'
3'… CGATC▲G …5'
Source Actinobacillus suis NH
Optimal buffer SE-buffer Y + BSA
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
75500010025
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA (HindIII-digest)
Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol. Store at -20°C
Ligation After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer Y, BSA
Notes To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Dedkov, V.S., Degtyarev, S.Kh. Biol. Chem. 379: 573-574 (1998).