SibEnzyme restriction enzymes database

Список ферментов

Mox20 I

Enzyme name Mox20 I
Prototype BalI
SKU SE-E301
Turbo version Not available
High-concentration version Not available
Recognition site
5'… TGG▼CCA …3'
3'… ACC▲GGT …5'
Source Microbacterium oxydans
Optimal buffer SE-buffer O
Optimal temperature 37 °C
Inactivation temperature не инактивируется
Buffer activity
BGOWYROSE
1025100752575
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lanbda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA (dcm-)
Storage conditions 10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 20-fold overdigestion with enzyme 80% of the DNA fragments can be ligated and recut. More complete
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Cleaved of DNA is impaired by overlapping Dcm methylation(C m CWGG) TGG CCA GG.
Supplied with enzyme 10 X SE-buffer O
Notes
References
  1. Chernov, A.P., Belichenko, O.A., Rechkunova, N.I., Andreeva, I.S., Repin, V.E., Degtyarev, S.K. Russian Patent Office (1993). SU 1806191 A3 Abdurashitov M.A., Tomilov V.N., Chernukhin V.A., Gonchar D. A., Degtyarev S. Kh. Comparative analysis of human chromosomal DNA digestion with restriction endonucleases in vitro and in silico. // Translated from "Medical genetics" V.6, No 8, pp 29-36, 2007

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Mox20 I

Enzyme name Mox20 I
Prototype BalI
SKU SE-E301
Turbo version Not available
High-concentration version Not available
Recognition site
5'… TGG▼CCA …3'
3'… ACC▲GGT …5'
Source Microbacterium oxydans
Optimal buffer SE-buffer O
Optimal temperature 37 °C
Inactivation temperature не инактивируется
Buffer activity
BGOWYROSE
1025100752575
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lanbda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA (dcm-)
Storage conditions 10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 20-fold overdigestion with enzyme 80% of the DNA fragments can be ligated and recut. More complete
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Cleaved of DNA is impaired by overlapping Dcm methylation(C m CWGG) TGG CCA GG.
Supplied with enzyme 10 X SE-buffer O
Notes
References
  1. Chernov, A.P., Belichenko, O.A., Rechkunova, N.I., Andreeva, I.S., Repin, V.E., Degtyarev, S.K. Russian Patent Office (1993). SU 1806191 A3 Abdurashitov M.A., Tomilov V.N., Chernukhin V.A., Gonchar D. A., Degtyarev S. Kh. Comparative analysis of human chromosomal DNA digestion with restriction endonucleases in vitro and in silico. // Translated from "Medical genetics" V.6, No 8, pp 29-36, 2007