SibEnzyme restriction enzymes database

Список ферментов

Rig I

Enzyme name Rig I
Prototype FseI
SKU SE-E529
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GGCCGG▼CC …3'
3'… CC▲GGCCGG …5'
Source Rhizobium yangligense
Optimal buffer SE-buffer RigI
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
75500105010
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Ad2 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Adenovirus -2 DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Storage at -20°C. Storage at -70°C is recommended for periods longer than 7 days.
Ligation After 3-fold overdigestion with enzyme > 95% of Ad2 DNA fragments can be ligated with T4 DNA Ligase and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Ad2 DNA with 4 units of enzyme for 16 hours at 37°C.
Methylation sensitivity Blocked by mCG or GmC methylation 5`-GGC(m5C)GGCC-3`/3`-CCGG(m5C)CGG-5` or 5`-GG(m5C)CGG(m5C)C-3`/3`-C(m5C)GGC(m5C)GG-5`
Supplied with enzyme 10 X SE-buffer RigI, BSA
Notes Do not use BSA for long incubation. To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.

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Rig I

Enzyme name Rig I
Prototype FseI
SKU SE-E529
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GGCCGG▼CC …3'
3'… CC▲GGCCGG …5'
Source Rhizobium yangligense
Optimal buffer SE-buffer RigI
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
75500105010
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Ad2 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Adenovirus -2 DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Storage at -20°C. Storage at -70°C is recommended for periods longer than 7 days.
Ligation After 3-fold overdigestion with enzyme > 95% of Ad2 DNA fragments can be ligated with T4 DNA Ligase and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Ad2 DNA with 4 units of enzyme for 16 hours at 37°C.
Methylation sensitivity Blocked by mCG or GmC methylation 5`-GGC(m5C)GGCC-3`/3`-CCGG(m5C)CGG-5` or 5`-GG(m5C)CGG(m5C)C-3`/3`-C(m5C)GGC(m5C)GG-5`
Supplied with enzyme 10 X SE-buffer RigI, BSA
Notes Do not use BSA for long incubation. To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.