SibEnzyme restriction enzymes database

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BslF I

Enzyme name BslF I
Prototype FinI
SKU SE-E479
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GGGAC(N)10▼ …3'
3'… CCCTG(N)14▲ …5'
Source Bacillus stearothermophilus Fl
Optimal buffer SE-buffer Y + BSA
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
2525102510050
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20°C.
Ligation After 3-fold overdigestion with enzyme 90% of DNA fragments can be ligated and 95% of these can be recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 2 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer Y, BSA
Notes High enzyme concentration may result in star activity. Long incubation with BSA is not recommend. To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml. There is DNA-methyltransferase activity in presence of SAM. It is maximum at 48°C. In presence of 10mM MgCl 2 enzyme both modifies and hydrolyzes DNA. If MgCl 2 is absent enzyme modifies DNA only. And that DNA become proof against BslFI. BslF I also cleaves the sequence GGGAC(11/15).
References
  1. Nadeev, A.N., Kashirina, J.G., Nayakshina, T.N., Dedkov, V.S., Mezentseva, N.V., Tomilova, J.E., Degtyarev, S.K.; Unpublished observations. 2004

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BslF I

Enzyme name BslF I
Prototype FinI
SKU SE-E479
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GGGAC(N)10▼ …3'
3'… CCCTG(N)14▲ …5'
Source Bacillus stearothermophilus Fl
Optimal buffer SE-buffer Y + BSA
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
2525102510050
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20°C.
Ligation After 3-fold overdigestion with enzyme 90% of DNA fragments can be ligated and 95% of these can be recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 2 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer Y, BSA
Notes High enzyme concentration may result in star activity. Long incubation with BSA is not recommend. To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml. There is DNA-methyltransferase activity in presence of SAM. It is maximum at 48°C. In presence of 10mM MgCl 2 enzyme both modifies and hydrolyzes DNA. If MgCl 2 is absent enzyme modifies DNA only. And that DNA become proof against BslFI. BslF I also cleaves the sequence GGGAC(11/15).
References
  1. Nadeev, A.N., Kashirina, J.G., Nayakshina, T.N., Dedkov, V.S., Mezentseva, N.V., Tomilova, J.E., Degtyarev, S.K.; Unpublished observations. 2004