SibEnzyme restriction enzymes database

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CciN I

Enzyme name CciN I
Prototype NotI
SKU SE-E203
Turbo version Available
High-concentration version Not available
Recognition site
5'… GC▼GGCCGC …3'
3'… CGCCGG▲CG …5'
Source Curtobacterium citreus N
Optimal buffer SE-buffer Y
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
25507575100100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Adenovirus-2 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Adenovirus-2 DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 5-fold overdigestion with enzyme about 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of DNA Ad-2 with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Blocked by CG methylation.
Supplied with enzyme 10 X SE-buffer Y.
Notes High enzyme concentration may result in star activity.
References
  1. Verchozina, V.A., Degtyarev, S.Kh. Gene 157: 99-100 (1995)

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CciN I

Enzyme name CciN I
Prototype NotI
SKU SE-E203
Turbo version Available
High-concentration version Not available
Recognition site
5'… GC▼GGCCGC …3'
3'… CGCCGG▲CG …5'
Source Curtobacterium citreus N
Optimal buffer SE-buffer Y
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
25507575100100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Adenovirus-2 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Adenovirus-2 DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 5-fold overdigestion with enzyme about 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of DNA Ad-2 with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Blocked by CG methylation.
Supplied with enzyme 10 X SE-buffer Y.
Notes High enzyme concentration may result in star activity.
References
  1. Verchozina, V.A., Degtyarev, S.Kh. Gene 157: 99-100 (1995)