SibEnzyme restriction enzymes database

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Bsp13 I

Enzyme name Bsp13 I
Prototype BspMII
SKU SE-E183
Turbo version Not available
High-concentration version Not available
Recognition site
5'… T▼CCGGA …3'
3'… AGGCC▲T …5'
Source Bacillus species 13
Optimal buffer SE-buffer 2K
Optimal temperature 50 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
2550755005
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dam-) in 1 hour at 50°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA (dam-)
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 50°C.
Methylation sensitivity Blocked by overlapping Dam methylation (G m ATC) TCCG GA TC and GA TC CGGA.
Supplied with enzyme 10 X SE-buffer 2K
Notes
References
  1. Dedkov, V.S., Rechkunova, N.I., Degtyarev, S.Kh. Unpublished observations.

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Bsp13 I

Enzyme name Bsp13 I
Prototype BspMII
SKU SE-E183
Turbo version Not available
High-concentration version Not available
Recognition site
5'… T▼CCGGA …3'
3'… AGGCC▲T …5'
Source Bacillus species 13
Optimal buffer SE-buffer 2K
Optimal temperature 50 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
2550755005
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dam-) in 1 hour at 50°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA (dam-)
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 50°C.
Methylation sensitivity Blocked by overlapping Dam methylation (G m ATC) TCCG GA TC and GA TC CGGA.
Supplied with enzyme 10 X SE-buffer 2K
Notes
References
  1. Dedkov, V.S., Rechkunova, N.I., Degtyarev, S.Kh. Unpublished observations.