SibEnzyme restriction enzymes database

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PalA I

Enzyme name PalA I
Prototype AscI
SKU SE-E483
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GG▼CGCGCC …3'
3'… CCGCGC▲GG …5'
Source An E. coli strain, that carries the cloned gene from Pseudomanas alcaligenes BS17
Optimal buffer SE-buffer Y
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
2510101010040
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and 95% of these can be recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Blocked by CG methylation.
Supplied with enzyme 10 X SE-buffer Y
Notes
References
  1. Dedkov, V.S., Belichenko, O.A., Gonchar, D.A., Akishev, A.G., Yamkovaya, T.V., Mezentseva, N.V., Tomilova, J.E., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations(2005)

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PalA I

Enzyme name PalA I
Prototype AscI
SKU SE-E483
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GG▼CGCGCC …3'
3'… CCGCGC▲GG …5'
Source An E. coli strain, that carries the cloned gene from Pseudomanas alcaligenes BS17
Optimal buffer SE-buffer Y
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
2510101010040
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and 95% of these can be recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Blocked by CG methylation.
Supplied with enzyme 10 X SE-buffer Y
Notes
References
  1. Dedkov, V.S., Belichenko, O.A., Gonchar, D.A., Akishev, A.G., Yamkovaya, T.V., Mezentseva, N.V., Tomilova, J.E., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations(2005)