SibEnzyme restriction enzymes database

Список ферментов

PciS I

Enzyme name PciS I
Prototype SapI
SKU SE-E497
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GCTCTTCN▼ …3'
3'… CGAGAAG(N)4▲ …5'
Source Planococcus citreus S
Optimal buffer SE-buffer B
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
10050007550
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA and 50% glycerol; Store at -20°C.
Ligation After 3-fold overdigestion with enzyme 90% of the DNA fragments can be ligated with T4 DNA Ligase at 16°C and 95% of these can be recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 0.5 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer B
Notes
References
  1. Chernukhin, V.A., Kileva, E.V., Tomilova, J.E., Dedkov, V.S. Unpublished observations (2006).

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PciS I

Enzyme name PciS I
Prototype SapI
SKU SE-E497
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GCTCTTCN▼ …3'
3'… CGAGAAG(N)4▲ …5'
Source Planococcus citreus S
Optimal buffer SE-buffer B
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
10050007550
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA and 50% glycerol; Store at -20°C.
Ligation After 3-fold overdigestion with enzyme 90% of the DNA fragments can be ligated with T4 DNA Ligase at 16°C and 95% of these can be recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 0.5 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer B
Notes
References
  1. Chernukhin, V.A., Kileva, E.V., Tomilova, J.E., Dedkov, V.S. Unpublished observations (2006).