SibEnzyme restriction enzymes database

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BstDS I

Enzyme name BstDS I
Prototype DsaI
SKU SE-E083
Turbo version Not available
High-concentration version Not available
Recognition site
5'… C▼CRYGG …3'
3'… GGYRC▲C …5'
Source Bacillus stearothermophilus DS
Optimal buffer SE-buffer Y
Optimal temperature 65 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
0755025100100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 65°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 10-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 65°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer Y
Notes
References
  1. Belichenko, O.A., Shevchenko, A.V., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations (1995).

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BstDS I

Enzyme name BstDS I
Prototype DsaI
SKU SE-E083
Turbo version Not available
High-concentration version Not available
Recognition site
5'… C▼CRYGG …3'
3'… GGYRC▲C …5'
Source Bacillus stearothermophilus DS
Optimal buffer SE-buffer Y
Optimal temperature 65 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
0755025100100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 65°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 10-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 65°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer Y
Notes
References
  1. Belichenko, O.A., Shevchenko, A.V., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations (1995).