SibEnzyme restriction enzymes database

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Hpa I

Enzyme name Hpa I
Prototype HpaI
SKU SE-E077
Turbo version Available
High-concentration version Not available
Recognition site
5'… GTT▼AAC …3'
3'… CAA▲TTG …5'
Source An E.coli strain, that carries the cloned gene Hpa I from Haemophilus parainfluenzae
Optimal buffer SE-buffer Y
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
050102510025
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 5-fold overdigestion with enzyme about 60% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer Y.
Notes High enzyme concentration results in star activity.
References
  1. Sharp, P.A., Sugden, B., Sambrook, J. Biochemistry 12: 3055-3063 (1973). Garfin, D.E., Goodman, H.M. Biochem. Biophys. Res. Commun. 59: 108-116 (1974).

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Hpa I

Enzyme name Hpa I
Prototype HpaI
SKU SE-E077
Turbo version Available
High-concentration version Not available
Recognition site
5'… GTT▼AAC …3'
3'… CAA▲TTG …5'
Source An E.coli strain, that carries the cloned gene Hpa I from Haemophilus parainfluenzae
Optimal buffer SE-buffer Y
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
050102510025
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 5-fold overdigestion with enzyme about 60% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer Y.
Notes High enzyme concentration results in star activity.
References
  1. Sharp, P.A., Sugden, B., Sambrook, J. Biochemistry 12: 3055-3063 (1973). Garfin, D.E., Goodman, H.M. Biochem. Biophys. Res. Commun. 59: 108-116 (1974).