SibEnzyme restriction enzymes database

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SfaN I

Enzyme name SfaN I
Prototype SfaNI
SKU SE-E165
Turbo version Available
High-concentration version Not available
Recognition site
5'… GCATC(N)5▼ …3'
3'… CGTAG(N)9▲ …5'
Source An E.coli strain that carries the cloned SfaN I gene from Streptococcus faecalis N
Optimal buffer SE-buffer O
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
102510075025
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 300 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 10-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer O
Notes
References
  1. Schildkraut, I., Greenough, L. Unpublished observations. V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006

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SfaN I

Enzyme name SfaN I
Prototype SfaNI
SKU SE-E165
Turbo version Available
High-concentration version Not available
Recognition site
5'… GCATC(N)5▼ …3'
3'… CGTAG(N)9▲ …5'
Source An E.coli strain that carries the cloned SfaN I gene from Streptococcus faecalis N
Optimal buffer SE-buffer O
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
102510075025
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 300 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 10-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer O
Notes
References
  1. Schildkraut, I., Greenough, L. Unpublished observations. V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006