SibEnzyme restriction enzymes database

Список ферментов

Mly113 I

Enzyme name Mly113 I
Prototype NarI
SKU SE-E189
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GG▼CGCC …3'
3'… CCGC▲GG …5'
Source Micrococcus lylae 113
Optimal buffer SE-buffer B
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
10025101050100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on T7 DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 3-fold overdigestion with enzyme more than 80% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of T7 DNA with 2 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer B
Notes High enzyme concentration may result in star activity.
References
  1. Dedkov, V.S., Zernov, Y.P., Rechkunova, N.I., Degtyarev, S.Kh. Mol.Genet.Mikrobiol.Virusol. 12: 17-18 (1990).

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Mly113 I

Enzyme name Mly113 I
Prototype NarI
SKU SE-E189
Turbo version Not available
High-concentration version Not available
Recognition site
5'… GG▼CGCC …3'
3'… CCGC▲GG …5'
Source Micrococcus lylae 113
Optimal buffer SE-buffer B
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
10025101050100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on T7 DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 3-fold overdigestion with enzyme more than 80% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of T7 DNA with 2 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer B
Notes High enzyme concentration may result in star activity.
References
  1. Dedkov, V.S., Zernov, Y.P., Rechkunova, N.I., Degtyarev, S.Kh. Mol.Genet.Mikrobiol.Virusol. 12: 17-18 (1990).