SibEnzyme restriction enzymes database

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Fsp4H I

Enzyme name Fsp4H I
Prototype Fnu4HI
SKU SE-E095
Turbo version Available
High-concentration version Not available
Recognition site
5'… GC▼NGC …3'
3'… CGN▲CG …5'
Source An E.coli strain that carries the cloned Fsp4H I gene from Flavobacterium species 4H
Optimal buffer SE-buffer Y
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
50751025100100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 200ug/ml BSA; 1mM DTT; 50% glycerol; Store at -20°C.
Ligation After 5-fold overdigestion with enzyme about 5% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Blocked by methylation: 5`-G(5mC)NGC-3`/ 3`-CGN(5mC)G-5`.
Supplied with enzyme 10 X SE-buffer Y
Notes
References
  1. Dedkov, V.S., Kileva, E.V., Shevchenko, A.V., Degtyarev, S.K. Unpublished observations (1995). Chmuzh E.V., Kashirina Yu.G., Tomilova Yu.E., Chernukhin V.A., Okhapkina S.S., Gonchar D. A., Dedkov V.S., Abdurashitov M. A., Degtyarev S. Kh. Molecular Biology, V.41, No 1, pp 43-50 (2007) (In Russian) Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.3, No 4, pp 19-27, 2007 V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006

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Fsp4H I

Enzyme name Fsp4H I
Prototype Fnu4HI
SKU SE-E095
Turbo version Available
High-concentration version Not available
Recognition site
5'… GC▼NGC …3'
3'… CGN▲CG …5'
Source An E.coli strain that carries the cloned Fsp4H I gene from Flavobacterium species 4H
Optimal buffer SE-buffer Y
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
50751025100100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 200ug/ml BSA; 1mM DTT; 50% glycerol; Store at -20°C.
Ligation After 5-fold overdigestion with enzyme about 5% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Blocked by methylation: 5`-G(5mC)NGC-3`/ 3`-CGN(5mC)G-5`.
Supplied with enzyme 10 X SE-buffer Y
Notes
References
  1. Dedkov, V.S., Kileva, E.V., Shevchenko, A.V., Degtyarev, S.K. Unpublished observations (1995). Chmuzh E.V., Kashirina Yu.G., Tomilova Yu.E., Chernukhin V.A., Okhapkina S.S., Gonchar D. A., Dedkov V.S., Abdurashitov M. A., Degtyarev S. Kh. Molecular Biology, V.41, No 1, pp 43-50 (2007) (In Russian) Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.3, No 4, pp 19-27, 2007 V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006