SibEnzyme restriction enzymes database

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Abs I

Enzyme name Abs I
Prototype AbsI
SKU SE-E535
Turbo version Not available
High-concentration version Not available
Recognition site
5'… CC▼TCGAGG …3'
3'… GGAGCT▲CC …5'
Source An E.coli strain that carries the cloned Abs I gene fromArthrobacter species 7М06
Optimal buffer SE-buffer AbsI
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
10100251025
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of pUC19SE/DriI - digest in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on pUC19SE/DriI - digest
Storage conditions 10 mM Tris-HCl (pH 7.5), 50 mM KCl, 0.1 mM EDTA, 7 mM 2-mercaptoethanol, 200 µg/ml BSA, 50% glycerol; Store at -20°C.
Ligation After 5-fold overdigestion with enzyme about 90% of the DNA fragments can be ligated with T4 DNA Ligase at 16°C and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer AbsI
Notes A long incubation time may result in star activity.
References
  1. V.A. Chernukhin, Yu.G. Kashirina, Yu.E. Tomilova, D.A. Gonchar, V.S. Dedkov, N.A. Mikhnenkova, S.Kh. Degtryarev. New Eight Bases Cutter Abs I from Arthrobacter species Recognizes Palindromic DNA Sequence 5’-CC^TCGAGG-3’ // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 2, pp 29-34, 2006

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Abs I

Enzyme name Abs I
Prototype AbsI
SKU SE-E535
Turbo version Not available
High-concentration version Not available
Recognition site
5'… CC▼TCGAGG …3'
3'… GGAGCT▲CC …5'
Source An E.coli strain that carries the cloned Abs I gene fromArthrobacter species 7М06
Optimal buffer SE-buffer AbsI
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
10100251025
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of pUC19SE/DriI - digest in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on pUC19SE/DriI - digest
Storage conditions 10 mM Tris-HCl (pH 7.5), 50 mM KCl, 0.1 mM EDTA, 7 mM 2-mercaptoethanol, 200 µg/ml BSA, 50% glycerol; Store at -20°C.
Ligation After 5-fold overdigestion with enzyme about 90% of the DNA fragments can be ligated with T4 DNA Ligase at 16°C and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer AbsI
Notes A long incubation time may result in star activity.
References
  1. V.A. Chernukhin, Yu.G. Kashirina, Yu.E. Tomilova, D.A. Gonchar, V.S. Dedkov, N.A. Mikhnenkova, S.Kh. Degtryarev. New Eight Bases Cutter Abs I from Arthrobacter species Recognizes Palindromic DNA Sequence 5’-CC^TCGAGG-3’ // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 2, pp 29-34, 2006