SibEnzyme restriction enzymes database

Список ферментов

Aox I

Enzyme name Aox I
Prototype AoxI
SKU SE-E569
Turbo version Not available
High-concentration version Not available
Recognition site
5'… ▼RG(5mC)Y …3'
3'… ▲RG(5mC)Y …5'
Source Arthrobacter oxydans 25K
Optimal buffer SE-buffer AoxI
Optimal temperature 60 °C
Inactivation temperature не инактивируется
Buffer activity
BGOWYROSE
7525102575100
Unit definition One unit is defined as the amount of enzyme required to hydrolyze in 1 μg of linearized plasmid pMHaeIII/DriI in 1 hour at 60°C in a total reaction volume of 50 μl. As a result a linearized plasmid pMHaeIII/DriI disappears (see run 4 in the figure). AoxI activity assay on DNA pMHaeIII/DriI Lanes: 1 - Control DNA pMHaeIII/DriI, 2 - 0.5 μl Aox I 3 - 1 μl Aox I 4 - 2 μl Aox I 5 - 1 Kb SE DNA-markers Products were separated in 1% agarose gel in Buffer TAE.
Assayed on DNA pMHaeIII/DriI is a linearized plasmid pMHaeIII. pMHaeIII carries a gene of DNA-methyltransferase M.HaeIII, which methylates sites 5`-GGCC-3` producing 5`-GG(5mC)C-3`/3`-C(5mC)GG-5`.
Storage conditions 10 mM Tris-HCl (pH 7.4); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethan. Store at -20°C.
Ligation
Nonspecific hydrolysis No detectable degradation of 1μg of Lambda DNA was observed after incubation with 1 units of enzyme for 16 hours at 60°C in a total reaction volume of 50 μl.
Methylation sensitivity The enzyme cleaves C5-methylated DNA and doesn't cut unmodified DNA.
Supplied with enzyme 10 X SE-buffer AoxI
Notes
References
  1. Chernukhin V.A., Belichenko O.A., Tarasova M.V., Gonchar D.A., Akishev A.G., Dedkov V.S., Mikhnenkova N.A., Degtyarev S.Kh. Bacterial strain Arthrobacter oxydans producer of AoxI site specific endonuclease. // Russian Federation patent RU 2399663 C1 (2009).

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Aox I

Enzyme name Aox I
Prototype AoxI
SKU SE-E569
Turbo version Not available
High-concentration version Not available
Recognition site
5'… ▼RG(5mC)Y …3'
3'… ▲RG(5mC)Y …5'
Source Arthrobacter oxydans 25K
Optimal buffer SE-buffer AoxI
Optimal temperature 60 °C
Inactivation temperature не инактивируется
Buffer activity
BGOWYROSE
7525102575100
Unit definition One unit is defined as the amount of enzyme required to hydrolyze in 1 μg of linearized plasmid pMHaeIII/DriI in 1 hour at 60°C in a total reaction volume of 50 μl. As a result a linearized plasmid pMHaeIII/DriI disappears (see run 4 in the figure). AoxI activity assay on DNA pMHaeIII/DriI Lanes: 1 - Control DNA pMHaeIII/DriI, 2 - 0.5 μl Aox I 3 - 1 μl Aox I 4 - 2 μl Aox I 5 - 1 Kb SE DNA-markers Products were separated in 1% agarose gel in Buffer TAE.
Assayed on DNA pMHaeIII/DriI is a linearized plasmid pMHaeIII. pMHaeIII carries a gene of DNA-methyltransferase M.HaeIII, which methylates sites 5`-GGCC-3` producing 5`-GG(5mC)C-3`/3`-C(5mC)GG-5`.
Storage conditions 10 mM Tris-HCl (pH 7.4); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethan. Store at -20°C.
Ligation
Nonspecific hydrolysis No detectable degradation of 1μg of Lambda DNA was observed after incubation with 1 units of enzyme for 16 hours at 60°C in a total reaction volume of 50 μl.
Methylation sensitivity The enzyme cleaves C5-methylated DNA and doesn't cut unmodified DNA.
Supplied with enzyme 10 X SE-buffer AoxI
Notes
References
  1. Chernukhin V.A., Belichenko O.A., Tarasova M.V., Gonchar D.A., Akishev A.G., Dedkov V.S., Mikhnenkova N.A., Degtyarev S.Kh. Bacterial strain Arthrobacter oxydans producer of AoxI site specific endonuclease. // Russian Federation patent RU 2399663 C1 (2009).