SibEnzyme restriction enzymes database

Список ферментов

Ars I

Enzyme name Ars I
Prototype ArsI
SKU SE-E575
Turbo version Not available
High-concentration version Not available
Recognition site
5'… ▼(N)8GACNNNNNNTTYG(N)11▼ …3'
3'… ▲(N)13CTGNNNNNNAARC(N)6▲ …5'
Source Arthrobacter species NTS
Optimal buffer SE-buffer Y
Optimal temperature 30 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
000010020
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 30°C in a total reaction volume of 50 μl.
Assayed on T7 DNA
Storage conditions 10 mM KH 2 PO 4 (pH 7.4); 200 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA, 50% glycerol. Store at -20°C.
Ligation After 3-fold overdigestion with enzyme 70% of DNA fragments can be ligated. Of these 80% can be recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of T7 DNA with 1 u.a. of enzyme for 16 hours at 30°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer Y, BSA
Notes To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.

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Ars I

Enzyme name Ars I
Prototype ArsI
SKU SE-E575
Turbo version Not available
High-concentration version Not available
Recognition site
5'… ▼(N)8GACNNNNNNTTYG(N)11▼ …3'
3'… ▲(N)13CTGNNNNNNAARC(N)6▲ …5'
Source Arthrobacter species NTS
Optimal buffer SE-buffer Y
Optimal temperature 30 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
000010020
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 30°C in a total reaction volume of 50 μl.
Assayed on T7 DNA
Storage conditions 10 mM KH 2 PO 4 (pH 7.4); 200 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA, 50% glycerol. Store at -20°C.
Ligation After 3-fold overdigestion with enzyme 70% of DNA fragments can be ligated. Of these 80% can be recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of T7 DNA with 1 u.a. of enzyme for 16 hours at 30°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer Y, BSA
Notes To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.