SibEnzyme restriction enzymes database

Список ферментов

Bar I

Enzyme name Bar I
Prototype BarI
SKU SE-E547
Turbo version Not available
High-concentration version Not available
Recognition site
5'… ▼(N)7GAAGNNNNNNTAC(N)12▼ …3'
3'… ▲(N)12CTTCNNNNNNATG(N)7▲ …5'
Source Bacillus sphaericus
Optimal buffer SE-buffer 2K
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
0025501040
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on T7 DNA
Storage conditions 20 mM KH 2 PO 4 (pH 7.4); 100 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA, 50% glycerol. Store at -20°C.
Ligation After 2-fold overdigestion with enzyme 90% of DNA fragments can be ligated. Of these 95% can be recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of T7 DNA with 2 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer 2K
Notes

Поиск по названию

Bar I

Enzyme name Bar I
Prototype BarI
SKU SE-E547
Turbo version Not available
High-concentration version Not available
Recognition site
5'… ▼(N)7GAAGNNNNNNTAC(N)12▼ …3'
3'… ▲(N)12CTTCNNNNNNATG(N)7▲ …5'
Source Bacillus sphaericus
Optimal buffer SE-buffer 2K
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
0025501040
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on T7 DNA
Storage conditions 20 mM KH 2 PO 4 (pH 7.4); 100 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA, 50% glycerol. Store at -20°C.
Ligation After 2-fold overdigestion with enzyme 90% of DNA fragments can be ligated. Of these 95% can be recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of T7 DNA with 2 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer 2K
Notes