SibEnzyme restriction enzymes database

Список ферментов

Bgl II

Enzyme name Bgl II
Prototype BglII
SKU SE-E027
Turbo version Available
High-concentration version Not available
Recognition site
5'… A▼GATCT …3'
3'… TCTAG▲A …5'
Source An E.coli strain that carries the cloned Bgl II gene from Bacillus globigii
Optimal buffer SE-buffer O
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
0101002510100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Not blocked by overlapping dam methylation (G m ATC) A GATC T
Supplied with enzyme 10 X SE-buffer O.
Notes
References
  1. Pirrotta, V. Nucleic Acids Res. 3: 1747-1760 (1976). Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.3, No 4, pp 19-27, 2007

Поиск по названию

Bgl II

Enzyme name Bgl II
Prototype BglII
SKU SE-E027
Turbo version Available
High-concentration version Not available
Recognition site
5'… A▼GATCT …3'
3'… TCTAG▲A …5'
Source An E.coli strain that carries the cloned Bgl II gene from Bacillus globigii
Optimal buffer SE-buffer O
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
0101002510100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Not blocked by overlapping dam methylation (G m ATC) A GATC T
Supplied with enzyme 10 X SE-buffer O.
Notes
References
  1. Pirrotta, V. Nucleic Acids Res. 3: 1747-1760 (1976). Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.3, No 4, pp 19-27, 2007