SibEnzyme restriction enzymes database

Список ферментов

BspFN I

Enzyme name BspFN I
Prototype FnuDII
SKU SE-E557
Turbo version Not available
High-concentration version Not available
Recognition site
5'… CG▼CG …3'
3'… GC▲GC …5'
Source Bacillus species FN
Optimal buffer SE-buffer Y
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
50757550100100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 20 mM Tris-HCl (pH 7.5); 300 mM NaCl; 0.1 mM EDTA; 200 μg/ml BSA, 7 mM 2-mercaptoethanol and 50% glycerol; Store at -20°C.
Ligation After 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Blocked by CG methylation
Supplied with enzyme 10 X SE-buffer Y
Notes

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BspFN I

Enzyme name BspFN I
Prototype FnuDII
SKU SE-E557
Turbo version Not available
High-concentration version Not available
Recognition site
5'… CG▼CG …3'
3'… GC▲GC …5'
Source Bacillus species FN
Optimal buffer SE-buffer Y
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
50757550100100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 20 mM Tris-HCl (pH 7.5); 300 mM NaCl; 0.1 mM EDTA; 200 μg/ml BSA, 7 mM 2-mercaptoethanol and 50% glycerol; Store at -20°C.
Ligation After 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity Blocked by CG methylation
Supplied with enzyme 10 X SE-buffer Y
Notes