SibEnzyme restriction enzymes database

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BstX I

Enzyme name BstX I
Prototype BstXI
SKU SE-E465
Turbo version Not available
High-concentration version Not available
Recognition site
5'… CCANNNNN▼NTGG …3'
3'… GGTN▲NNNNNACC …5'
Source Bacillus stearothermophilus X
Optimal buffer SE-buffer O
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
10101007525100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer O
Notes
References
  1. Wise, R., Schildkraut, I. Unpublished observations (1992)

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BstX I

Enzyme name BstX I
Prototype BstXI
SKU SE-E465
Turbo version Not available
High-concentration version Not available
Recognition site
5'… CCANNNNN▼NTGG …3'
3'… GGTN▲NNNNNACC …5'
Source Bacillus stearothermophilus X
Optimal buffer SE-buffer O
Optimal temperature 37 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
10101007525100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer O
Notes
References
  1. Wise, R., Schildkraut, I. Unpublished observations (1992)