SibEnzyme restriction enzymes database

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Cci I

Enzyme name Cci I
Prototype BspHI
SKU SE-E565
Turbo version Available
High-concentration version Not available
Recognition site
5'… T▼CATGA …3'
3'… AGTAC▲T …5'
Source Curtobacterium citreus
Optimal buffer SE-buffer W
Optimal temperature 55 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
0102510075100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 20-fold overdigestion with enzyme about 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of DNA with 40 u.a. of enzyme for 16 hours at 55°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer W, BSA
Notes Do not use BSA for long incubation To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.

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Cci I

Enzyme name Cci I
Prototype BspHI
SKU SE-E565
Turbo version Available
High-concentration version Not available
Recognition site
5'… T▼CATGA …3'
3'… AGTAC▲T …5'
Source Curtobacterium citreus
Optimal buffer SE-buffer W
Optimal temperature 55 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
0102510075100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 20-fold overdigestion with enzyme about 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of DNA with 40 u.a. of enzyme for 16 hours at 55°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer W, BSA
Notes Do not use BSA for long incubation To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.