SibEnzyme restriction enzymes database

Список ферментов

Fai I

Enzyme name Fai I
Prototype FaiI
SKU SE-E551
Turbo version Not available
High-concentration version Not available
Recognition site
5'… YA▼TR …3'
3'… RT▲AY …5'
Source Flavobacterium aquatile B15 FaiI cleaves 4 expected recognition sites as well as several other sites with a weaker activity. In the case of long incubation with FaiI DNA can be digested to small oligos.
Optimal buffer SE-buffer B
Optimal temperature 50 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
10050102525100
Unit definition One unit is defined as the amount of enzyme required to cleave 1 pmol of the double-stranded oligonucleotide of the above indicated structure in 1 hour at 50°C in a total reaction volume of 20 μl.
Assayed on Double-stranded oligonucleotide 5`- CGAGTTCA^TAGCTGGGCCCAAC -3` 3`- GCTCAAGT^ATCGACCCGGGTTG -5`
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 3-fold overdigestion with enzyme about 90% of the pUC19 DNA fragments can be ligated with DNA Ligase and recut.
Nonspecific hydrolysis
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer B [[HDR:Non-specific hydrolisis]]:
Notes
References
  1. Tarasova G.V., Chernukhin V.A., Tomilova J.E., Degtyarev S.Kh Substrate specificity of new restriction endonuclease FaiI // In press

Поиск по названию

Fai I

Enzyme name Fai I
Prototype FaiI
SKU SE-E551
Turbo version Not available
High-concentration version Not available
Recognition site
5'… YA▼TR …3'
3'… RT▲AY …5'
Source Flavobacterium aquatile B15 FaiI cleaves 4 expected recognition sites as well as several other sites with a weaker activity. In the case of long incubation with FaiI DNA can be digested to small oligos.
Optimal buffer SE-buffer B
Optimal temperature 50 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
10050102525100
Unit definition One unit is defined as the amount of enzyme required to cleave 1 pmol of the double-stranded oligonucleotide of the above indicated structure in 1 hour at 50°C in a total reaction volume of 20 μl.
Assayed on Double-stranded oligonucleotide 5`- CGAGTTCA^TAGCTGGGCCCAAC -3` 3`- GCTCAAGT^ATCGACCCGGGTTG -5`
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 3-fold overdigestion with enzyme about 90% of the pUC19 DNA fragments can be ligated with DNA Ligase and recut.
Nonspecific hydrolysis
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer B [[HDR:Non-specific hydrolisis]]:
Notes
References
  1. Tarasova G.V., Chernukhin V.A., Tomilova J.E., Degtyarev S.Kh Substrate specificity of new restriction endonuclease FaiI // In press