SibEnzyme restriction enzymes database

Список ферментов

Gsa I

Enzyme name Gsa I
Prototype BseYI
SKU SE-E563
Turbo version Not available
High-concentration version Not available
Recognition site
5'… CCCAG▼C …3'
3'… G▲GGTCG …5'
Source Geobacillus stearothermophilus Y
Optimal buffer SE-buffer W
Optimal temperature 70 °C
Inactivation temperature не инактивируется
Buffer activity
BGOWYROSE
10257510075100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 70°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 20-fold overdigestion with enzyme about 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 70°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer W, BSA
Notes Do not use BSA for long incubation. To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
References
  1. Dedkov V.S., Mikhnenkova N.A., Kileva E.V., Tarasova G.V., Gonchar D.A., Akishev A.G., Chernukhin V.A., Shkirja S.S., Degtyarev S.Kh. New restriction endonuclease GsaI, neoschizomer of BseY I, recognizes 5’-CCCAGC(-1/-5)-3’.// Bulletin of biotechnology and physico-chemical biology named by Yu.A.Ovchinnikov (Moscow), V.4, No.1, p.19-24 (2008). (In Russian)

Поиск по названию

Gsa I

Enzyme name Gsa I
Prototype BseYI
SKU SE-E563
Turbo version Not available
High-concentration version Not available
Recognition site
5'… CCCAG▼C …3'
3'… G▲GGTCG …5'
Source Geobacillus stearothermophilus Y
Optimal buffer SE-buffer W
Optimal temperature 70 °C
Inactivation temperature не инактивируется
Buffer activity
BGOWYROSE
10257510075100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 70°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligation After 20-fold overdigestion with enzyme about 90% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 70°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer W, BSA
Notes Do not use BSA for long incubation. To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
References
  1. Dedkov V.S., Mikhnenkova N.A., Kileva E.V., Tarasova G.V., Gonchar D.A., Akishev A.G., Chernukhin V.A., Shkirja S.S., Degtyarev S.Kh. New restriction endonuclease GsaI, neoschizomer of BseY I, recognizes 5’-CCCAGC(-1/-5)-3’.// Bulletin of biotechnology and physico-chemical biology named by Yu.A.Ovchinnikov (Moscow), V.4, No.1, p.19-24 (2008). (In Russian)