SibEnzyme restriction enzymes database

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Hind III

Enzyme name Hind III
Prototype HindIII
SKU SE-E073
Turbo version Available
High-concentration version Available
Recognition site
5'… A▼AGCTT …3'
3'… TTCGA▲A …5'
Source An E.coli strain, that carries the cloned gene Hind III from Haemophilus influenzae Rd
Optimal buffer SE-buffer W
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
102501000100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 50-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer W, BSA
Notes To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Old, R., Murray, K., Roizes, G. J. Mol. Biol. 92: 331-339 (1975). V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006 HindIII

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Hind III

Enzyme name Hind III
Prototype HindIII
SKU SE-E073
Turbo version Available
High-concentration version Available
Recognition site
5'… A▼AGCTT …3'
3'… TTCGA▲A …5'
Source An E.coli strain, that carries the cloned gene Hind III from Haemophilus influenzae Rd
Optimal buffer SE-buffer W
Optimal temperature 37 °C
Inactivation temperature 80 °C
Buffer activity
BGOWYROSE
102501000100
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on Lambda DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligation After 50-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer W, BSA
Notes To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Old, R., Murray, K., Roizes, G. J. Mol. Biol. 92: 331-339 (1975). V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006 HindIII