SibEnzyme restriction enzymes database

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Psr I

Enzyme name Psr I
Prototype PsrI
SKU SE-E131
Turbo version Not available
High-concentration version Not available
Recognition site
5'… ▼(N)7GAACNNNNNNTAC(N)12▼ …3'
3'… ▲(N)12CTTGNNNNNNATG(N)7▲ …5'
Source Pseudomonas stutzeri N2
Optimal buffer SE-buffer Y
Optimal temperature 30 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
10100010030
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 30°C in a total reaction volume of 50 μl.
Assayed on T7 DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA, 50% glycerol. Store at -20°C.
Ligation After 2-fold overdigestion with enzyme more than 70% of DNA fragments can be ligated. Of these 80% can be recut. In the presence of 10% PEG
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of T7 DNA with 2 u.a. of enzyme for 16 hours at 30°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer Y, BSA
Notes High enzyme concentration may result in star activity. Incubation at 37°C results in 20% activity. To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Dedkov, V.S., Kileva, E.V., Abdurashitov, M.A., Gonchar, D.A., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2001).

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Psr I

Enzyme name Psr I
Prototype PsrI
SKU SE-E131
Turbo version Not available
High-concentration version Not available
Recognition site
5'… ▼(N)7GAACNNNNNNTAC(N)12▼ …3'
3'… ▲(N)12CTTGNNNNNNATG(N)7▲ …5'
Source Pseudomonas stutzeri N2
Optimal buffer SE-buffer Y
Optimal temperature 30 °C
Inactivation temperature 65 °C
Buffer activity
BGOWYROSE
10100010030
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 30°C in a total reaction volume of 50 μl.
Assayed on T7 DNA
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA, 50% glycerol. Store at -20°C.
Ligation After 2-fold overdigestion with enzyme more than 70% of DNA fragments can be ligated. Of these 80% can be recut. In the presence of 10% PEG
Nonspecific hydrolysis No nonspecific activity was detected after incubation of 1 μg of T7 DNA with 2 u.a. of enzyme for 16 hours at 30°C.
Methylation sensitivity not tested
Supplied with enzyme 10 X SE-buffer Y, BSA
Notes High enzyme concentration may result in star activity. Incubation at 37°C results in 20% activity. To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
References
  1. Dedkov, V.S., Kileva, E.V., Abdurashitov, M.A., Gonchar, D.A., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2001).